The effect of heparin 100IU and Na2EDTA in concentration of 3%, 5% and 10% on the blood coagulation and hematological parameters of hybrid surubim catfish were evaluated. We used ten fish weighing 386,7±24,3g and average length of 38,7±6,4cm for collection of blood samples and determination of the percentage of hematocrit, plasma total protein content and osmotic fragility erythrocytes test (OFE). Data were submitted to analysis of variance and averages compared by Tukey test at 5% probability. Coagulation was efficiently inhibited when used the Na2EDTA, while the samples with heparin coagulate ten hours after collection. There was an increase in osmotic fragility of erythrocytes (P<0.01), especially when using Na2EDTA 10% and 5%, with no difference between control and heparin. In hematocrit and total plasma protein content were not observed statistical differences. The Na2EDTA 3% is safe and effective anticoagulant for hybrid surubim catfish, preventing clotting for more than 10 hours causing slight effect on the osmotic fragility of erythrocytes.
anticoagulant; coagulation; fish hematology; Pseudoplatystoma sp
