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Population densities of Ralstonia solanacearum on potato cultivars in the field

The occurrence of latent populations of Ralstonia solanacearum on potato (Solanum tuberosum) plants may represent inoculum source of unknown potential. Besides, it is also unknown if the latent population of the bacterium is lower in resistant than in susceptible cultivars. In order to study these points, an experiment was conducted in the field in two locations in Rio Grande do Sul State, Brazil. The objective was to verify the relationship between R. solanacearum population density and the potato cultivar resistance. The experiment was conducted in Eldorado do Sul, during the Spring, and in Caxias do Sul, during the Fall. Tubers of Achat, Baronesa, Elvira, Macaca, Monte Bonito, and Trapeira cultivars were inoculated with a R. solanacearum strain, biovar II, and planted in the field. Population density of the bacterium was estimated in the assymptomatic and wilted plants, by ELISA and imunofluorescence techniques. No evidence of relationship between population density of R. solanacearum and cultivar was found. Besides, the population density on Achat, known as the most resistant, one was similar to the other cultivars.

bacterial wilt; Solanum tuberosum; ELISA; imunofluorescence


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