Orthophytum mucugense is an ornamental bromeliad endemic to the municipality of Mucugê-BA which is vulnerable to extinction. The aim of this study was to establish a regeneration protocol via direct organogenesis for the species. The explants root, stem and leaf from plants of different ages were inoculated in the MS½ culture medium supplemented with BAP and NAA. The shoots were inoculated on medium supplemented with IBA and activated charcoal. The plants were transferred to a substrate composed of vermiculite and soil. The highest rate of sprouting was obtained with stem explants of 20 and 40 days old in medium supplemented with 0.65mM of NAA. For rooting the presence of activated charcoal had a positive influence on the length of shoot and root plants system. The plants were acclimatized with 100% survival.
Bromeliaceae; tissue culture; micropropagation; ornamental plants
