Effects of storage on vis-NIR-SWIR reflectance spectra of Mombasa grass leaf samples

Tavares, Tiago Rodrigues; Fiorio, Peterson Ricardo; Seixas, Hugo Tameirão; Garcia, Amparo Cisneiros; Barros, Pedro Paulo da Silva

doi:10.1590/0103-8478cr20190587

Brasil

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BIOLOGY • Cienc. Rural 50 (3) • 2020 • https://doi.org/10.1590/0103-8478cr20190587 linkcopy

Effects of storage on vis-NIR-SWIR reflectance spectra of Mombasa grass leaf samples

Efeitos do armazenamento no espectro de reflectância vis-NIR-SWIR de amostras foliares de capim Mombaça

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

Vis-NIR-SWIR reflectance spectra of leaf samples, collected in the laboratory, allow the calibration of predictive models to quantify their physicochemical attributes in a practical manner and without producing chemical residues. This technique should enable the development of management strategies for intensification of pasture use. However, spectral analysis performed in the laboratory may be affected by the deterioration of plant material during transport from the field to the lab, so storage methods are necessary. This research aimed to evaluate the effects of different storage methods on the spectral response of Mombasa grass leaves. Three methods were evaluated: (i) artificially refrigerated environment, (ii) humid environment, and (iii) without microenvironment control. These methods were tested in five different storage times: 2 hours, 4 hours, 8 hours, 24 hours and 48 hours. The spectral behavior of the leaves still inserted in the plant was used as a quality reference. Results showed notable changes at the earliest storage time for the treatment without microenvironment control. Both methods with microenvironment control stabilized the occurrence of spectral changes over 48 hours of the samples storage, thus both were suggested for this species.

Key words: diffuse reflectance spectroscopy; hyperspectral sensors; forage; precision agriculture

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Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br

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[TFN1] Means followed by equal letters (vertical) do not differ statistically from each other by the Tukey test at 5%.

Storage method	--------------------------------------Central wavelength of the spectral range (nm)-------------------------------
	480	550	670	740	910	1450	1940
G	4.85 b	13.51 b	4.74 b	40.78 b	44.19 b	10.05 a	2.47 a
A	6.03 a	14.73 ab	5.69 a	42.25 b	45.61 b	10.28 a	2.94 ab
AL	6.70 a	15.84 a	6.38 a	43.8 a	47.37 a	11.31 a	3.95 a

Storage method	--------------------------------------Storage periods----------------------------					Linear reg	Quad. reg.
	T2	T4	T8	T24	T48
-------------------------------------------------------------------------------- 480 nm ---------------------------------------------------------------------------
G	1.89 b	2.42 a	1.48 a	1.82 a	1.57 b	ns	ns
A	1.24 b	2.90 a	0.39 a	0.23 b	1.32 b	ns	ns
AL	3.47 a	3.28 a	0.98 a	2.56 a	4.32 a	ns	ns
------------------------------------------------------------------------------- 550 nm ----------------------------------------------------------------------------
G	2.43 ab	3.17 a	2.91 a	3.16 a	2.33 a	ns	ns
A	1.26 b	4.29 a	1.92 a	1.57 ab	4.74 a	ns	ns
AL	3.57 a	2.55 a	-1.11 a	0.22 b	2.97 a	ns	ns
------------------------------------------------------------------------------- 670 nm ----------------------------------------------------------------------------
G	1.75 b	2.37 a	1.45 a	1.70 a	1.61 b	ns	ns
A	1.26 b	3.15 a	0.49 a	0.38 b	1.88 b	ns	ns
AL	3.42 a	3.13 a	0.81 a	2.20 a	3.94 a	ns	ns
------------------------------------------------------------------------------ 740 nm -----------------------------------------------------------------------------
G	1.99 ab	3.25 ab	3.27 a	3.88 a	2.87 a	ns	ns
A	0.99 b	4.49 a	1.93 ab	1.99 a	2.62 a	ns	ns
AL	3.10 a	2.23 b	0.62 b	1.96 a	3.47 a	ns	ns
----------------------------------------------------------------------------- 910 nm ------------------------------------------------------------------------------
G	0.64 b	1.89 a	1.87 ab	2.81 b	2.12 b	ns	ns
A	- 0.14 b	3.22 a	0.50 b	0.80 c	0.84 b	ns	ns
AL	2.40 a	2.12 a	2.06 a	8.59 a	9.93 a	<0.01	<0.01
--------------------------------------------------------------------------- 1450 nm ----------------------------------------------------------------------------
G	3.96 b	4.00 b	1.07 b	2.25 b	1.98 b	ns	ns
A	4.07 b	3.82 b	0.48 b	0.59 b	0.44 b	ns	ns
AL	6.00 a	8.60 a	10.30 a	32.57 a	35.46 a	<0.01	<0.01
----------------------------------------------------------------------------1940 nm ------------------------------------------------------------------------------
G	2.56 b	2.96 b	0.49 b	0.79 b	0.59 b	ns	ns
A	2.81 b	4.10 ab	-0.06 b	-0.09 b	0.00 b	ns	ns
AL	4.10 a	5.15 a	4.17 a	23.39 a	27.20 a	<0.01	<0.01

------------------------------------------------------------------------------------G------------------------------------------------------------------------------
Storage periods	--------------------------------------------Central wavelength of the spectral range (nm)------------------------------------
	480	550	670	740	910	1450	1940
0	4.85 b	13.51 b	4.73 b	40.78 b	44.19 a	10.05 b	2.47 b
T2	6.73 a	15.94 a	6.49 a	42.77 a	44.83 a	14.01 a	5.03 a
-------------------------------------------------------------------------------------A-------------------------------------------------------------------------------
Storage periods	-------------------------------------------Central wavelength of the spectral range (nm)-------------------------------------
	480	550	670	740	910	1450	1940
0	6.03 b	14.73 b	5.69 b	42.25 b	45.61 a	10.28 b	2.94 b
T2	7.27 a	15.96 a	6.95 a	43.23 a	45.47 a	14.36 a	5.75 a
-------------------------------------------------------------------------------------AL-----------------------------------------------------------------------------
Storage periods	-------------------------------------------Central wavelength of the spectral range (nm)-------------------------------------
	480	550	670	740	910	1450	1940
0	6.70 b	15.85 b	6.38 b	43.84 b	47.37 b	11.31 b	3.95 b
T2	10.17 a	19.42 a	9.79 a	46.94 a	49.76 a	17.31 a	8.05 a