The objective of this work was to obtain a shooting regeneration protocol from explants of pear tree (Pyrus communis L.) Carrick cultivar, aiming at genetic transformation programs. Three types of explants (caulinary tip, leaf and entrenode) were used. They were left for 0, 10, 20, 30 or 40 days in induction medium, and afterwards they were transferred to proliferation medium with 8.9 or 13.3µM of TDZ. At 85 days after the beginning of the experiment, it was noted that the caulinary tip explant showed higher capacity of shooting regeneration when compared to entrenode explants, while the leaf explant was not able to regenerate. It was also observed that inoculating the explant directly in proliferation medium without auxin resulted in a higher percentage of regeneration, and increasing the period of time that the explants remained in induction medium lowered the percentage of regeneration of shoots. The TDZ concentration in the proliferation medium did not affect the capacity of shooting regeneration.
tissue culture; organogenesis; thidiazuron
