An in vitro method was developed for the establishment and regeneration of larger numbers of uniform plants from the basal parts of the flower of Gerbera jamesonii. The culture medium was MS (MURASHIGE & SKOOG, 1962) solidified with 0.7% agar and supplemented with adenine (80mg/l), tyrosine (100mg/l) and different concentrations and combinations of BAP (6-benzylaminopurine) with IAA (indoleacetic acid), and IAA with 2.4-D (2.4-dichlorophenoxyacetic). Multiple shoot buds formation is observed from capitulum on MS medium incorporate with 3, 6, and 9mg/l of BAP. At 3mg/l of BAP two shoot bud formation per explant are regenerate, at 6mg/l of BAP three shoots and at 9mg/l of BAP just one. 2.4-D is not necessary at this stage of culture establishment.
tissue culture; growth regulator; Gerbera jamesonii
