Four inactivated vaccines formulated with a bovine herpesvirus tipe 5 (BHV-5) strain isolated from an outbreak of bovine meningoencephalitis in Rio Grande do Sul, Brazil, were administred in forty cattle as to evaluate its immunogenicity. The vaccines A and B used mineral oil and vaccines C and D used aluminum hydroxide (Al2(OH)3) as adjuvant. The titer of the viral suspension used in all vaccines was 10(7.50) TCID50 / 25 mul. Immunostin®, a Mycobacterium derived immunostimulant, was added to vaccines B and D. After receiving three doses of vaccines, at 30 days intervals, only the animals of the groups A (90%) and B (100%) developed significant antibody titers. The responses to these vaccines were adjusted to linear regression, showing that the antiboby titers increased progressively with the number of immunizations. The use of Immunostin® enhanced the immunogenicity of the vaccine with aluminum hidroxide but was ineffective when associated with oil adjuvants. It was concluded that inactivated BHV-5 vaccines prepared with high titer viral suspensions and oil adjuvants induce adequate levels of antibodies in a high proportion of the vaccinees after the third dose.
bovine herpesvirus 1 and 5; BHV- 5; BHV-1; vaccines
