The viability of a homologous diaphragmatic muscle preserved in 98% glycerin for cervical tracheal repair in dogs was studied. Eighteen adult mongrel dogs, eight males, weighting 6 to 21kg were used. Five tracheal rings (2.0 x 4.5cm) were partially resected and a homologous muscle fragment was implanted. The animals were distributed in three numerally equal groups. They were observed for a post-operative period of 15 days (group I), 30 days (group II) and 60 days (group III). After these periods, the dogs were reoperated for macroscopic evaluation and collection of samples for histological evaluations. Tracheal repair with progressive substitution of the muscle flap by fibrous connective tissue, epithelial migration through the tracheal lumen and presence of cilia were detected. The homologous diaphragmatic muscle fragment preserved in 98% glycerin acts as a scaffold for the growth of fibrous connective tissue and allows epithelialization across the implant, without exuberant proliferation. Thus it can be used for the repair of tracheal defects in dogs.
graft; diaphragmatic; surgery; healing; dog
