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Different substrates and environment conditions on in vitro rooting of blueberry

The aim of this research was to compare the in vitro rooting ability of blueberry (Vaccinium ashei Reade cv. 'Delite' and V. corymbosum L. cv. 'Georgiagem'), to study the effect of alternative substrate instead of agar (perlite, vermiculite and agar + activated charcoal) added to the WPM culture medium containing 7µM L-1 of AIB, and the light effect by raising the explants in different growth environment: conventional growth room and greenhouse. The use of perlite followed by vermiculite increased roots number and length, total fresh weight and root weight and rooting percentage. The addition of activated charcoal inhibited rooting. The environment condition did not alter the number of formed roots. The cv. 'Georgiagem' showed the highest percentages of rooting whether compared cv. 'Delite', independently of growth environment and substrate.

Vaccinium ashei; V. corymbosum; natural light; activated charcoal; perlite; vermiculite


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