With reference to the controversy regarding animal experimentation, this article questions the relationship with the professional training in universities. The considerations voiced in the paper are based on data gathered from interviews with students and professors of Biological Science courses at two different universities in São Paulo. These data reveal that the use of animals is not seen as an ethical problem by most of the people interviewed and, also, that there exists a high degree of unfamiliarity with the laws, principles and techniques recommended for animal experimentation. It is also suggested that this situation springs from a class orientation and predominance of a technician tendency geared toward professionalization, which in turn is subordinated to market demands, in university teaching.