Circ 0006282/miR-155 reduced inflammation in diabetic nephropathy via expression of SIRT1/NLRP3 signaling pathway

This study aimed to explore the effects and mechanism of circ 0006282 in Diabetic nephropathy (DN) in vitro and in vivo. 6-week-old male db/db mice were induced by intraperitoneal (I.P) injection of STZ (50 mg/kg, S0130, Sigma, St. Louis, MO, USA) for five consecutive days. HK-2 cells was incubated with high glucose for vitro model. Western blotting, immunostaining, and biochemical assays were used to obtain that inflammatory factor and protein expressions. The current study demonstrated that the serum circ 0006282 expression were reduced, and serum miR-155 expression in patients with DN were increased. Circ 0006282 reduced inflammation in kidney cell by high glucose. MiR-155 promoted inflammation in kidney cell by high glucose. Circ 0006282 reduced inflammation via activation of SIRT1/NLRP3 signaling pathway by suppression of miR-155 expression. MiR-155 regulated the anti-inflammation effects of circ 0006282 on inflammation in kidney cell by high glucose by SIRT1/NLRP3 signaling pathway. Taken together, these results suggest that circ 0006282 may represent promising strategy for the therapy of DN.

Keywords:
circ 0006282; diabetic nephropathy; miR-155; SIRT1; NLRP3

Authorship

Dan WANG ^**Corresponding author: may80311@163.com

Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaSouthern Medical UniversityChinaGuangzhou, Guangdong, ChinaDepartment of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China

https://orcid.org/0000-0002-5134-5418

Zikun ZHANG

Zekun SI

Ling WANG

*Corresponding author: may80311@163.com

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (7)

Thumbnail

Figure 1
Circ 0006282 and miR-155 expression in DN mice. Circ 0006282 and miR-155 expression in DN mice (A and B), between circ 0006282 and miR-155 (C), between miR-155 and creatinine (D), between circ 0006282 and creatinine (E); luciferase reporter activity (F), circ 0006282 binding site miRNA-155 (G), over-expression of circ 0006282 reduced miR-155 expression (H), and down-regulation of circ 0006282 promoted miR-155 expression (I). Sham, normal volunteers group; DN, DN patients group; ^##p<0.01 compared with DN patients group; p<0.01 compared with DN patients group.

Thumbnail

Figure 2
Circ 0006282 reduced inflammation in kidney cell by high glucose. Circ 0006282 expression (A), TNF-α, IL-1β, IL-6 and IL-18 cell levels (B, C, D and E) by over-expression of circ 0006282; Circ 0006282 expression (F), TNF-α, IL-1β, IL-6 and IL-18 cell levels (G, H, I and J) by down-rgulation of circ 0006282. Negative, negative mimics group; Circ 0006282, over-expression of circ 0006282 group; Circ 0006282 inhibitor, down-regulation of circ 0006282 group. ^##p<0.01 compared with negative mimics group.

Thumbnail

Figure 3
miR-155 regulated inflammation via SIRT1/NLRP3 signaling pathway. Heat map and analysis results of gene chip (A and B), Volcano Plot (C), analysis results (D), network of SIRT1/NLRP3 signaling pathway (E).

Thumbnail

Figure 4
Circ 0006282 regulated inflammation via SIRT1/NLRP3 signaling pathway by miR-155. Luciferase reporter activity (A), SIRT1 binding site miRNA-155 (B), SIRT1/NLRP3 signaling pathway by over-expression of miR-155 (C, D and E), SIRT1/NLRP3 signaling pathway by down-regulation of miR-155 (F, G and H), SIRT1/NLRP3 signaling pathway by down-regulation of Circ 0006282 (I, J and K), SIRT1/NLRP3 signaling pathway by over-expression of Circ 0006282 (L, M and N). Negative, negative mimics group; miR-155, over-expression of miR-155 group; miR-155 inhibitor, down-regulation of miR-155 group; Circ 0006282, over-expression of circ 0006282 group; Circ 0006282 inhibitor, down-regulation of circ 0006282 group. ^##p<0.01 compared with negative mimics group.

Thumbnail

Figure 5
MiR-155 promoted inflammation in kidney cell by high glucose. MiR-155 expression (A), TNF-α, IL-1β, IL-6 and IL-18 cell levels (B, C, D and E) by over-expression of miR-155; miR-155 expression (F), TNF-α, IL-1β, IL-6 and IL-18 cell levels (G, H, I and J) by down-rgulation of miR-155. Negative, negative mimics group; miR-155, over-expression of miR-155 group; miR-155 inhibitor, down-regulation of miR-155 group. ^##p<0.01 compared with negative mimics group.

Thumbnail

Figure 6
MiR-155 regulated the anti-inflammation effects of circ 0006282 on inflammation in kidney cell by high glucose by SIRT1/NLRP3 signaling pathway. MiR-155 expression (A), SIRT1/NLRP3 signaling pathway (B, C and D), TNF-α, IL-1β, IL-6 and IL-18 cell levels (E, F, G and H). Negative, negative mimics group; Circ 0006282, over-expression of circ 0006282 group; Circ 0006282+ miR-155, over-expression of circ 0006282 and miR-155 group. ^##p<0.01 compared with negative mimics group; **p<0.01 compared with over-expression of circ 0006282 group.

Thumbnail

Figure 7
MiR-155 inhibitor regulated the effects of circ 0006282 inhibitor on inflammation in kidney cell by high glucose by SIRT1/NLRP3 signaling pathway.MiR-155 expression (A), SIRT1/NLRP3 signaling pathway (B, C and D), TNF-α, IL-1β, IL-6 and IL-18 cell levels (E, F, G and H). Negative, negative mimics group; Circ 0006282 inhibitor, down-regulation of circ 0006282 group; Circ 0006282 inhibitor+miR-155 inhibitor, down-regulation of circ 0006282 and miR-155 group. ^##p<0.01 compared with negative mimics group; **p<0.01 compared with down-regulation of circ 0006282 group.

Figure 1 Circ 0006282 and miR-155 expression in DN mice. Circ 0006282 and miR-155 expression in DN mice (A and B), between circ 0006282 and miR-155 (C), between miR-155 and creatinine (D), between circ 0006282 and creatinine (E); luciferase reporter activity (F), circ 0006282 binding site miRNA-155 (G), over-expression of circ 0006282 reduced miR-155 expression (H), and down-regulation of circ 0006282 promoted miR-155 expression (I). Sham, normal volunteers group; DN, DN patients group; ^##p<0.01 compared with DN patients group; p<0.01 compared with DN patients group.

Figure 2 Circ 0006282 reduced inflammation in kidney cell by high glucose. Circ 0006282 expression (A), TNF-α, IL-1β, IL-6 and IL-18 cell levels (B, C, D and E) by over-expression of circ 0006282; Circ 0006282 expression (F), TNF-α, IL-1β, IL-6 and IL-18 cell levels (G, H, I and J) by down-rgulation of circ 0006282. Negative, negative mimics group; Circ 0006282, over-expression of circ 0006282 group; Circ 0006282 inhibitor, down-regulation of circ 0006282 group. ^##p<0.01 compared with negative mimics group.

Figure 3 miR-155 regulated inflammation via SIRT1/NLRP3 signaling pathway. Heat map and analysis results of gene chip (A and B), Volcano Plot (C), analysis results (D), network of SIRT1/NLRP3 signaling pathway (E).

Figure 4 Circ 0006282 regulated inflammation via SIRT1/NLRP3 signaling pathway by miR-155. Luciferase reporter activity (A), SIRT1 binding site miRNA-155 (B), SIRT1/NLRP3 signaling pathway by over-expression of miR-155 (C, D and E), SIRT1/NLRP3 signaling pathway by down-regulation of miR-155 (F, G and H), SIRT1/NLRP3 signaling pathway by down-regulation of Circ 0006282 (I, J and K), SIRT1/NLRP3 signaling pathway by over-expression of Circ 0006282 (L, M and N). Negative, negative mimics group; miR-155, over-expression of miR-155 group; miR-155 inhibitor, down-regulation of miR-155 group; Circ 0006282, over-expression of circ 0006282 group; Circ 0006282 inhibitor, down-regulation of circ 0006282 group. ^##p<0.01 compared with negative mimics group.

Figure 5 MiR-155 promoted inflammation in kidney cell by high glucose. MiR-155 expression (A), TNF-α, IL-1β, IL-6 and IL-18 cell levels (B, C, D and E) by over-expression of miR-155; miR-155 expression (F), TNF-α, IL-1β, IL-6 and IL-18 cell levels (G, H, I and J) by down-rgulation of miR-155. Negative, negative mimics group; miR-155, over-expression of miR-155 group; miR-155 inhibitor, down-regulation of miR-155 group. ^##p<0.01 compared with negative mimics group.

Figure 6 MiR-155 regulated the anti-inflammation effects of circ 0006282 on inflammation in kidney cell by high glucose by SIRT1/NLRP3 signaling pathway. MiR-155 expression (A), SIRT1/NLRP3 signaling pathway (B, C and D), TNF-α, IL-1β, IL-6 and IL-18 cell levels (E, F, G and H). Negative, negative mimics group; Circ 0006282, over-expression of circ 0006282 group; Circ 0006282+ miR-155, over-expression of circ 0006282 and miR-155 group. ^##p<0.01 compared with negative mimics group; **p<0.01 compared with over-expression of circ 0006282 group.

Figure 7 MiR-155 inhibitor regulated the effects of circ 0006282 inhibitor on inflammation in kidney cell by high glucose by SIRT1/NLRP3 signaling pathway.MiR-155 expression (A), SIRT1/NLRP3 signaling pathway (B, C and D), TNF-α, IL-1β, IL-6 and IL-18 cell levels (E, F, G and H). Negative, negative mimics group; Circ 0006282 inhibitor, down-regulation of circ 0006282 group; Circ 0006282 inhibitor+miR-155 inhibitor, down-regulation of circ 0006282 and miR-155 group. ^##p<0.01 compared with negative mimics group; **p<0.01 compared with down-regulation of circ 0006282 group.

How to cite

copy

Sociedade Brasileira de Ciência e Tecnologia de Alimentos Av. Brasil, 2880, Caixa Postal 271, 13001-970 Campinas SP - Brazil, Tel.: +55 19 3241.5793, Tel./Fax.: +55 19 3241.0527 - Campinas - SP - Brazil
E-mail: revista@sbcta.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Circ 0006282/miR-155 reduced inflammation in diabetic nephropathy via expression of SIRT1/NLRP3 signaling pathway

PlumX

Mensagem

[1] *Corresponding author: may80311@163.com