Abstract
Effect of peptides obtained from Phaseolus lunatus L. where biological properties such as antioxidant activity have been found. In addition to improve this beneficial effect, the microencapsulation could be a way to protect the peptides against the environment to which they are exposed. Gums extracted from plant seeds are a potential option such as Guazuma ulmifolia, and its seeds gum exhibits promising properties as coating materials in encapsulation. Two peptide fractions from P. lunatus L. were encapsulated (>10 and <10 kDa) by ionic gelation using mixtures of G. ulmifolia gum and sodium alginate (GUG:SA). A 23 experimental design was used: GUG:SA ratios (A) (70:30 or 30:70); CaCl2 concentrations (B) (0.05 or 0.15 M); and hardening time (C). (10 or 30 min). Multiple variable response analysis with a desirability coefficient identified optimum conditions for each peptide fraction. Better results were obtained for >10 kDa peptide fraction at optimal conditions of A: 70:30, B: 0.05 and C: 10, obtaining irregular beads with a diameter of 5.85 mm2, Bead Encapsulation efficiency 42% and 31 and 42 mM TEAC for ABTS and DPPH respectively. These results shown that GUG:SA mixture is a viable encapsulation system for preserving antioxidant peptide fractions.
Keywords:
encapsulation; ionic gelation; alternative gum; bioactive properties; hydrolysates
