Mechanochemical-assisted extraction of polysaccharides from bamboo leaves and its optimized processing parameters

SHEN, Dan; JIN, Tingyu; WANG, Jianguo; ZHU, Xingyi

doi:10.1590/fst.117821

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Food Science and Technology

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Original Article • Food Sci. Technol 42 • 2022 • https://doi.org/10.1590/fst.117821 copy

Mechanochemical-assisted extraction of polysaccharides from bamboo leaves and its optimized processing parameters

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Mechanochemical-assisted extraction (MCAE) method was applied to the extraction of polysaccharides from the bamboo leaves. The MCAE parameters were investigated via response surface methodology design experiments, the processing conditions were optimized as follows: milling time 30 min, liquid-solid ratio 2:1, extraction time 48 min. Under these conditions, the experimental value of (11.40 ± 0.12)% corresponded with the value predicted by the model and was higher than super fine-grinding extraction (SFGE) of (11.17 ± 0.15)% and heat reflux extraction (HRE) of (10.92 ± 0.17)%. The mechanism of mechanochemical-assisted extraction (MCAE) was discussed based on scanning electron microscope. Compared to HRE, the extraction time was significantly shortened, the water consumption and extraction temperature were also lowered. MCAE is a good choice for the efficient extraction of polysaccharides from bamboo leaves.

Keywords:
mechanochemical-assisted extraction; polysaccharides; bamboo leaves; response surface methodology

Independent variables		levels
Independent variables	-1	0	1
Milling time (X₁) (min)	10	20	30
Liquid-solid ratio (X₂)	15:01	20:01	25:01:00
Extraction time (X₃) (min)	20	40	60

Run	X₁	X₂	X₃	Yield of polysaccharide (%)
Run	X₁	X₂	X₃	Actual values	Predicted values
1	1	0	1	10.87	11.03
2	0	1	-1	9.08	8.90
3	-1	-1	0	6.77	6.69
4	1	1	0	10.34	10.42
5	-1	0	1	9.42	9.32
6	0	0	0	11.07	11.32
7	0	-1	1	8.66	8.84
8	1	0	-1	8.89	8.99
9	0	0	0	11.45	11.32
10	-1	0	-1	7.51	7.35
11	0	0	0	11.22	11.32
12	-1	1	0	9.27	9.62
13	0	-1	-1	6.32	6.57
14	0	0	0	11.32	11.32
15	0	1	1	10.88	10.63
16	0	0	0	11.56	11.32
17	1	-1	0	9.58	9.23

variables	Sum of squares	df	Mean square	F value	p-value prob>F
Model	43.02	9	4.78	50.32	<0.0001
X₁	5.63	1	5.63	59.48	0.0001
X₂	8.49	1	8.49	89.70	<0.0001
X₃	8.06	1	8.06	85.18	<0.0001
X₁X₂	0.76	1	0.76	8.00	0.0255
X₁X₃	1.225E-003	1	1.225E-003	0. 0.013	0.9126
X₂X₃	0.073	1	0.073	0.77	0.4092
X₁²	3.79	1	3.79	40.01	0.0004
X₂²	8.09	1	8.09	85.45	<0.0001
X₃²	6.10	1	6.10	64.43	<0.0001
residual	0.66	7	0.095
Lack of fit	0.52	3	0.17	4.68	0.0851
Pure error	0.15	4	0.037
Cor total	43.68	16

Method	MCAE	SFGE	HRE
Solvent	Water	Water	Water
Extraction time (min)	48	48	120
Extraction temperature (°C)	60	60	80
Liquid-solid ratio (mL/mg)	21	21	25
Extraction times	1	1	2
Yield (%) (g/g)	11.40 ± 0.12	11.17 ± 0.15	10.92 ± 0.17
Content of polysaccharides (%)	31.12 ± 0.19	29.87 ± 0.23	30.11 ± 0.14

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[1] *Corresponding author: zhuxingyi3141@163.com