The effect of Guizhi decoction on inflammatory response induced by myocardial ischemia

Abstract

We aim to explore the influence of Guizhi decoction(GD) on inflammatory response in myocardial ischemia models. 45 New Zealand rabbits were divided into three groups randomly: sham control group, myocardial ischemia group and myocardial ischemia + Guizhi decoction treatment group. The rabbits were treated with Guizhi decoction or saline for 20 days. After that, the myocardial ischemia model was created by left anterior descending coronary artery ligation. Color Doppler Echocardiography computed the ejection fraction (EF). Serum content of MyD88 and TNF-α and concentration of cardiac markers (CK-MB, CK, LDH and cTnT) was measured. After ligation, ST segment was elevated. However, 15 rabbits died due to operation and postoperative care, and were excluded from this study. The final sample size was sham control group (n=10), myocardial ischemia group (n=10) and Guizhi decoction group (n=10) Compared with the sham control group, the ejection fraction was decreased and the expression of the TLR4, MyD88, TNF-α,CK-MB, CK, LDH and cTnT in myocardial ischemia group was increased (p<0.05). After treatment with Guizhi decoction, EF was improved and related inflammatory response factors were decreased (p <0.05). Pretreatment with Guizhi decoction attenuated the level of proinflammatory markers and improved heart function in animal model for myocardial ischemia.

Keywords:
myocardial ischemia; inflammatory response; Guizhi decoction; echocardiography