The aim of this work was to study the effects of different parameters in the production of β-1,3 glucanases, proteases and chitinases by Cellulosimicrobium celulans 191, in culture media A, B and C, respectively. The experimental designs employed were 2³ factorial designs, and the factors studied were: initial pH, temperature and rotary shaker speed. The experimental results for the production of β-1,3 glucanase in culture medium A showed maximum activity (0.64 U.mL-1) with an initial pH of 8.5 after 24 hours of fermentation at 33 ºC and 200 rpm. The parameters pH, rotary shaker speed and the interaction of all the parameters presented statistical significance. The experimental results for protease production in culture medium B showed maximum activity (4.25 U.mL-1) with an initial pH of 6.5 after 30 hours of fermentation at 20 ºC and 200 rpm. The pH was the only parameter that presented statistical significance. The experimental results for chitinase production in culture medium C showed maximum activity (7.06 U.mL-1) at an initial pH of 5.5 after 72 hours of fermentation at 25 ºC and 200 rpm. All the parameters and their interactions presented statistical significance. The determination coefficients of the analysis of variance and F tests demonstrated that the linear regression models obtained were significant at a confidence level of 95%.
β-1,3 glucanases; proteases; chitinases; Cellulosimicrobium cellulans 191
