Antiviral activities of <i>Radix Isatidis</i> polysaccharide against type II herpes simplex virus in vitro

WANG, Chunmei; RUAN, Shiman; GU, Xunmei; ZHU, Bin

doi:10.1590/1678-457X.19317

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Food Science and Technology

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Research Paper • Food Sci. Technol 38 (Suppl. 1) • Dec 2018 • https://doi.org/10.1590/1678-457X.19317 copy

Antiviral activities of Radix Isatidis polysaccharide against type II herpes simplex virus in vitro

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

This study investigated the antiviral activities of Radix Isatidis polysaccharide (RIP) against type II herpes simplex virus (HSV-2) in vitro. RIP was prepared from the Radix Isatidis root. The toxicity of RIP on Vero cells was detected. The direct killing effect of RIP on HSV-2, inhibitory effect of RIP on HSV-2 replication and inhibitory effect of RIP on HSV-2 adsorption were determined. Results showed that, RIP in concentration range of 25-800 mg/L had no toxic effect on Vero cells. RIP with different concentrations could not directly inactivate the HSV-2. The effective rates on inhibition of HSV-2 replication and adsorption in 800 mg/L RIP group were 71.57% and 48.37%, respectively, which were the highest among different groups. In conclusion, RIP has the antiviral effect against HSV-2 in vitro. This effect mainly occurs in inhibiting the virus duplication and adsorption.

Keywords:
Radix Isatidis; polysaccharide; HSV-2

Group	Dose (mg/L)	OD_570nm
Norma cells	-	0.49 ± 0.06
RIP	25	0.50 ± 0.04
	50	0.51 ± 0.07
	100	0.53 ± 0.07
	200	0.51 ± 0.08
	400	0.52 ± 0.06
	800	0.53 ± 0.08

Group	Dose (mg/L)	OD_570nm	CPE
Virus	-	0.38 ± 0.04^* * P < 0.05 compared with normal cell group.	+++
Norma cell	-	0.70 ± 0.07	-
RIP	25	0.36 ± 0.05^* * P < 0.05 compared with normal cell group.	+++
	50	0.37 ± 0.06^* * P < 0.05 compared with normal cell group.	+++
	100	0.38 ± 0.07^* * P < 0.05 compared with normal cell group.	+++
	200	0.40 ± 0.06^* * P < 0.05 compared with normal cell group.	+++
	400	0.39 ± 0.07^* * P < 0.05 compared with normal cell group.	+++
	800	0.40 ± 0.06^* * P < 0.05 compared with normal cell group.	+++

Group	Dose (mg/L)	OD_570nm	CPE	Effective rate (%)
Virus	-	0.31 ± 0.03^* * P < 0.05 compared with normal cell group;	+++
Norma cell	-	0.55 ± 0.09	-
RIP	25	0.32 ± 0.04^* * P < 0.05 compared with normal cell group;	+++	0.96
	50	0.32 ± 0.04^* * P < 0.05 compared with normal cell group;	+++	1.92
	100	0.34 ± 0.04^* * P < 0.05 compared with normal cell group;	+++	7.35
	200	0.50 ± 0.05^# # P < 0.05 compared with virus group.	++	39.3
	400	0.51 ± 0.04^# # P < 0.05 compared with virus group.	++	46.65
	800	0.54 ± 0.04^# # P < 0.05 compared with virus group.	+	71.57

Group	Dose (mg/L)	OD_570nm	CPE	Effective rate (%)
Virus	-	0.37 ± 0.04^* * P < 0.05 compared with normal cell group;	+++
Norma cell	-	0.56 ± 0.07	-
RIP	25	0.37 ± 0.05^* * P < 0.05 compared with normal cell group;	+++	0.82
	50	0.39 ± 0.05^* * P < 0.05 compared with normal cell group;	+++	5.43
	100	0.41 ± 0.05^* * P < 0.05 compared with normal cell group;	+++	12.50
	200	0.42 ± 0.05^* * P < 0.05 compared with normal cell group;	+++	13.86
	400	0.51 ± 0.05^# # P < 0.05 compared with virus group.	++	38.04
	800	0.55 ± 0.07^# # P < 0.05 compared with virus group.	+	48.37

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[1] *Corresponding author: zpb1375027@126.com