Correlation of the free radical and antioxidant activities of <i>Eriobotrya Japonica</i> Lindl. with phenolic and flavonoid contents

SEON, Ho Young; SUN, Sangouk; YIM, Soon-Ho

doi:10.1590/fst.21720

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Food Science and Technology

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Original Article • Food Sci. Technol (Campinas) 41 (4) • Oct-Dec 2021 • https://doi.org/10.1590/fst.21720 copy

Correlation of the free radical and antioxidant activities of Eriobotrya Japonica Lindl. with phenolic and flavonoid contents

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Abstract

This study was conducted to compare the correlations between antioxidant activity and free radicals. Total Polyphenol and Flavonoid contents were the highest 31.97 ± 1.29 mg TAE/g and 96.1 ± 0.31 mg CE/g in hot water extract, respectively. FRAP activity was highest at 559.17 ± 2.69 mg TE/g in 60% EtOH extract. The RC50 values of DPPH and ABTS radical scavenging activity were lowest at 61.96 ± 5.01 μg/mL in 80% EtOH, 73.81 ± 0.16 μg/mL in 60% EtOH, respectively. The RC50 values of hydrogen peroxide scavenging activity and NO radical scavenging activity were lowest at 265.33 ± 3.88 μg/mL in 60% EtOH and 52.51 ± 8.54 μg/mL in hot water extract, respectively. The total polyphenol and flavonoid contents had significant correlation with the free radical scavenging ability and electron donating ability. On the other hand, there was confirmed significant correlation of free radical and antioxidant activities.

Keywords:
Eriobotrya japonica; antioxidant; correlation; ROS; RNS

Sample	Yields (wt%)¹ 1) Yield (%, w/w) = (dry weight of extract / weight of dry E. japonica Lindl.) × 100; )	Polyphenol contents (mg TAE/g² 2) Total Polyphenol analyzed as tannic acid equivalent (TAE) mg/g of extract; ))	Flavonoid contents (mg CE/g³ 3) Total Flavonoid content analyzed as catechin equivalent (CE) mg/g of extract; values are the average of triplicates. ))
Water	20.00	31.97 ± 1.29⁴ 4) Values are means of triplicate determination ± S.D; ) c⁵ 5) Means with different letters (a-e) in the same column are significantly different at p<0.05 (Tukey) )	96.10 ± 0.31 e
20% EtOH	14.46	20.33 ± 0.02 a	73.20 ± 0.20 b
40% EtOH	5.22	26.50 ± 0.26 b	88.69 ± 0.20 d
60% EtOH	9.37	27.51 ± 0.71b	88.47 ± 0.26 d
80% EtOH	14.44	25.92 ± 1.79 b	81.79 ± 0.16 c
100% EtOH	9.16	19.11 ± 1.06 a	66.74 ± 0.16 a

Factors	TPC	TFC	FRAP	DPPH	ABTS	HP	NO
TPC	1	0.955^ p < 0.01 (Pearson). ¹ 1) Correlation is significantly different at )	0.859**	0.526**	0.485**	0.467**	0.193
TFC	-	1	0.916**	0.670**	0.654**	0.465**	0.350
FRAP² 2) FRAP (Ferric reducing antioxidant power) )	-	-	1	0.737**	0.707**	0.450*	0.403^* * p < 0.05 and ¹ 1) Correlation is significantly different at )
DPPH³ 3) DPPH (α, α-diphenyl-β-picrylhydrazyl) radical scavenging )	-	-	-	1	0.972**	0.801**	0.905**
ABTS⁴ 4) ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging )	-	-	-	-	1	0.874**	0.933**
HP⁵ 5) HP (Hydrogen peroxide) scavenging )	-	-	-	-	-	1	0.777**
NO⁶ 6) NO (Nitric oxide) radical scavenging )	-	-	-	-	-	-	1

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[1] *Corresponding author: virshyim@gmail.com