Extraction optimization of antioxidant polysaccharides from   <i>Auricularia auricula</i> fruiting bodies

Zou, Yu; Jiang, Aili; Tian, Mixia

doi:10.1590/1678-457X.6712

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Research Paper • Food Sci. Technol (Campinas) 35 (3) • Jul-Sep 2015 • https://doi.org/10.1590/1678-457X.6712 copy

Extraction optimization of antioxidant polysaccharides from Auricularia auricula fruiting bodies

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The extraction conditions (liquid-solid ratio, temperature and time) of antioxidant polysaccharides from Auricularia auricula fruiting bodies (AAFB) were optimized using response surface methodology (RSM). The Box-Behnken experimental results showed the optimum extraction conditions as follows: a liquid-solid ratio of 38.77 mL/g, a temperature of 93.98 °C and a time of 3.41 h. Under these conditions, the maximal polysaccharide yield was 10.46 g/100 g. In addition, AAFB polysaccharides exhibited stronger antioxidant activities by evaluating of Fe²⁺-chelating ability and hydroxyl radical scavenging activity with IC₅₀ values of 0.43 and 0.38 mg/mL, respectively. These results indicated that AAFB polysaccharides might be potentially used as a natural antioxidant.

Keywords:
Auricularia auricula ; polysaccharide; extraction; antioxidant activity

Run	A: Liquid-solid ratio (mL/g)	B: Temperature (°C)	C: Time (h)	Polysaccharide yield (g/100 g)
1	40	80	3	10.07±0.31
2	20	100	3	7.39±0.30
3	20	60	3	5.86±0.19
4	40	100	1	6.41±0.26
5	60	100	3	6.46±0.25
6	40	80	3	10.13±0.51
7	20	80	5	4.96±0.13
8	60	80	5	4.85±0.17
9	20	80	1	4.59±0.18
10	60	60	3	6.21±0.30
11	40	80	3	10.30±0.49
12	60	80	1	5.11±0.17
13	40	80	3	10.29±0.46
14	40	60	1	4.59±0.16
15	40	100	5	9.63±0.38
16	40	80	3	7.74±0.23
17	40	60	5	4.13±0.16

Source	Sum of squares	Degree of freedom	Mean squares	F-value	P-value
Model	73.61	9	8.18	5.90	0.0145
A	0.01	1	0.01	0.01	0.9607
B	10.35	1	10.35	7.46	0.0293
C	1.03	1	1.03	0.74	0.4174
A ²	21.68	1	21.68	15.63	0.0055
B ²	3.85	1	3.85	2.78	0.1394
C ²	27.58	1	27.58	19.89	0.0029
AB	0.41	1	0.41	0.30	0.6037
AC	0.09	1	0.09	0.07	0.7968
BC	3.39	1	3.39	2.44	0.1622
Residual	9.71	7	1.39
Lack of Fit	4.84	3	1.61	1.32	0.3836
Pure Error	4.87	4	1.22
Corrected Total	83.32	16		R² = 0.9135

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[1] *Corresponding author: yuzou126@126.com