Efficiency of γ-oryzanol against the complex <i>Fusarium graminearum</i> growth and mycotoxins production

BEMVENUTI, Renata; RODRIGUES, Marcy Heli; FURLONG, Eliana

doi:10.1590/fst.01818

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Food Science and Technology

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Original Article • Food Sci. Technol 39 (1) • 2019 • https://doi.org/10.1590/fst.01818 copy

Efficiency of γ-oryzanol against the complex Fusarium graminearum growth and mycotoxins production

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The ability of γ-oryzanol against Fusarium graminearum growth and mycotoxins production were assessed and compared with the antioxidant properties in order to improve its application in food chain, beyond of the conventional. The γ-oryzanol extracted from rice bran and characterized by HPLC-UV and GG-FD. Its antifungal in vitro, effect on complex F. graminearum CQ244 (halo diameter, ergosterol and glucosamine production) and mycotoxin production (deoxinivalenol, nivalenol and zearalenone) were determined by an adapted method adapted. The antifungal activity of γ-oryzanol was compared with azoxystrobin and trifloxystrobin. MIC and specific inhibitory effects (%inhibition µg γ-oryzanol^-1) were also estimated. The antioxidant activity of the γ-oryzanol (DPPH, FRAP, ABTS and enzymatic inhibition) was compared with the gallic acid. Considering the MIC (1, 2 mg.g^-1 ) specific inhibitory effects on fungal growth (0.045%.µg) and mycotoxin (DON 0.001%. µg and NIV 0.002%. ug). Therefore γ-oryzanol is also a potential natural antifungal that is able to difficult the synthesis of fungal cell wall.

Keywords:
deoxynivalenol; nivalenol; zearalenone; antioxidant properties

Antifungal Agent	antifungal	Halo		Glucosamine		Ergosterol
Antifungal Agent	antifungal	Mean ± SD (cm)	Inhibition (%)	Mean ± SD (mg/g)	Inhibition (%)	Mean ± SD (mg/g)	Inhibition (%)
Control	-	8.0 ± 0	-	31.0 ± 2.9	-	1.4 ± 0.3	-
Azoxystrobin	0.2 mg kg^-1	8.0 ± 0	0	16.0 ± 2.1	48	1.2 ± 0.1	15
Trifloxystrobin	0.4 mg kg^-1	6.9 ± 0	14	16.1 ± 3.4	48	1.3 ± 0.3	10
Oryzanol	0.1 g kg^-1	8.0 ± 0	0	19.2 ± 2.6	38	1.4 ± 0.2	1
Oryzanol	0.3 g kg^-1	8.0 ± 0	0	15.9 ± 3.1	49	0.9 ± 0.1	32
Oryzanol	0.6 g kg^-1	8.0 ± 0	0	13.2 ± 3.5	57	0.9 ± 0.1	35
Oryzanol	0.8 g kg^-1	8.0 ± 0	0	7.1 ± 0.0	77	0.9 ± 0.1	32
Oryzanol	1.0 g kg^-1	5.0 ± 0.9	37	20.7 ± 0.5	33	0.9 ± 0.1	36

Main active	Antifungal	Mycotoxins (µg kg^-1)
Main active	Antifungal	NIV	DON	ZEA
Control	-	84.59	115.11	70.89
Azoxystrobin	0.2 mg kg^-1	87.25	78.58	78.59
Trifloxystrobin	0.4 mg kg^-1	89.75	67.51	72.88
Oryzanol	0.1 g kg^-1	81.29	87.14	70.60
Oryzanol	0.3 g kg^-1	91.24	81.47	67.39
Oryzanol	0.6 g kg^-1	97.53	113.22	65.46
Oryzanol	0.8 g kg^-1	61.03	105.66	69.14
Oryzanol	1.0 g kg^-1	54.88	88.43	70.66

Specific inhibition	Azoxistrobine	Trifloxistrobine	γ-oryzanol
% Halo growth/µg antifungal	NI	0.014	0.04
% ergosterol/ µg antifungal	0.048	0.05	0.05
% glucosamine/ µg antifungal	0.015	0.010	0.04
% deoxynivalenol/ µg antifungal	0.001	0.0005	0.001
% nivalenol/ µg antifungal	NI	NI	0.002
% zearalenone/ µg antifungal	NI	NI	NI

antioxidants	γ-oryzanol standard	Gallic acid standard	γ-oryzanol Crude
DPPH	0.07 ± 0.01	14.2 ± 0.18	2.1 ± 0.22
mM rolox/ µg	0.14 ± 0.01	2.3 ± 0.07	0.07 ± 0.02
FRAP/ µg	0.39 ± 0.06	2591 ± 1.2	0.03 ± 0.00
Inibição %PO/ µg	0.96 ± 0.01	7.5 ± 0.12	0.5 ± 0.00

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[1] * Corresponding author: dqmebf@furg.br