Establishment of a mouse pneumonia model under cold stress

Abstract

Simulating the cold living environment of the human body in winter, establishing a mouse model of pneumonia under single-factor cold stimulation to provide a basis and a new direction for further research on the relationship between cold and pulmonary inflammation. C57/BL6J mice were divided into three groups: A (initial control group), B (control group), and C (experimental group). They were randomly divided into 10 mice for each group, placed in an environment (temperature 22 ± 1 °C, humidity 50 ± 5%). After 3 weeks, mice in group A were sacrificed. Group C mice were exposed to environment (temperature 4 °C, humidity 50 ± 5%) for 8 h (9 am to 5 pm), and mice in group B were placed. (The temperature was 22 ± 1°C, humidity 50 ± 5%). The lungs of the mice in group A and B were normal. The inflammatory cells infiltrated in the mice in group C, and the alveolar structure disappeared. The neutrophil immunohistochemistry showed a significant increase in neutrophils and the percentage of blood neutrophils. Higher than group A and B (P < 0.05). The mice were stimulated daily for 8 hours at a temperature of 4 °C and humidity (50 ± 5) % for 30 days to establish a cold-stimulated pneumonia model.

Keywords:
cold stimulation; pulmonary infection; model; mouse