Puerariae Flos extracts possess the potential antioxidant efficacy against oxidant stress

SEON, Ho-Young; JEON, So-Heon; YIM, Soon-Ho

doi:10.1590/fst.105221

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Food Science and Technology

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Original Article • Food Sci. Technol 42 • 2022 • https://doi.org/10.1590/fst.105221 copy

Puerariae Flos extracts possess the potential antioxidant efficacy against oxidant stress

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Abstract

In this study, yield of Puerariae Flos extract showed 60% ethanol gave the highest yield of 26.34%. Total polyphenol contents and total flavonoid contents were highest in 40% ethanol extract (5.86 mg TAE/g) and hot water extract (3.06 mg CE/g), respectively. Evaluation of the reducing power of transition metal ions such as phosphomolybdenum and ferric tripyridyltriazine complex were high in 100% ethanol extract (4.41 mg AAE/g) and hot water extract (42.62 µM TE/g), respectively. Evaluation of free radical scavenging activity such as DPPH, ABTS and NO were excellent in 40% ethanol extract (95.74 µM TE/g, 17.78 µM TE/g, 175.5 µg/mL) and the HP radical scavenging activity was excellent in 80% ethanol extract (308.3 µg/mL), respectively. A positive correlation (p < 0.01) was showed between TFC and FRAP assay (r = 0.866) highest significant. The correlation between PMA and ABTS radical scavenging activity was significantly high with r = 0.913 (p < 0.01). The overall results of this work showed that Puerariae Flos is a candidate natural source of antioxidants.

Keywords:
Puerariae Flos; total phenolic content; antioxidant activity

Sample	Yield¹ 1) Yield (%, w/w) = (dry weight of extracts / weight of dry Puerariae Flos) × 100. )	Polyphenol contents	Flavonoid contents
Sample	(%, w/w)	(mg TAE/g² 2) Tannic acid equivalent. ))	(mg CE/g³ 3) Catechin equivalent. ))
D.W	11.05	5.09 ± 0.01^b)⁴ 4) Values are mean ± SD (n=3). Means with different letters (a-f) in the same column are significantly different at p < 0.05 (Tukey). )	3.06 ± 0.02^e)
20% EtOH	22.30	4.94 ± 0.01^a)	3.02 ± 0.01^d)
40% EtOH	23.32	5.86 ± 0.01^f)	2.79 ± 0.01^c)
60% EtOH	26.34	5.43 ± 0.01^c)	2.59 ± 0.01^b)
80% EtOH	20.84	5.75 ± 0.01^d)	2.42 ± 0.01^a)
100% EtOH	9.45	5.82 ± 0.01^e)	2.43 ± 0.01^a)

Sample	PMA¹ 1) Phosphomolybdenum complex assay. )	FRAP² 2) Ferric-reducing antioxidant power. )	DPPH	ABTS
Sample	(mg AAE/g³ 3) Ascorbic acid equivalent. ))	(mM TE/g⁴ 4) Trolox equivalent. ))	(mM TE/g)	(mM TE/g)
D.W	1.54 ± 0.02^a)⁵ 5) Values are mean ± SD (n=3). Means with different letters (a-f) in the same column are significantly different at p < 0.05 (Tukey). )	42.62 ± 0.19^f)	92.66 ± 0.17^b)	17.04 ± 0.02^c)
20% EtOH	1.83 ± 0.00^b)	34.84 ± 0.51^d)	92.33 ± 0.17^b)	17.34 ± 0.02^b)
40% EtOH	1.82 ± 0.04^b)	36.94 ± 0.19^e)	95.74 ± 0.17^a)	17.78 ± 0.02^a)
60% EtOH	1.86 ± 0.03^b)	24.07 ± 0.00^c)	92.72 ± 0.14^b)	15.46 ± 0.02^d)
80% EtOH	2.50 ± 0.03^c)	22.40 ± 0.00^b)	91.86 ± 0.13^c)	14.61 ± 0.03^e)
100% EtOH	4.41 ± 0.02^d)	14.96 ± 0.19^a)	80.08 ± 0.21^d)	12.15 ± 0.03^f)

Sample	RC50¹ 1) The half reduction concentration. )
Sample	NO² 2) Nitric oxide radical scavenging activity. )	HP³ 3) Hydrogen peroxidescavenging activity. )
BHA⁴ 4) Positive control. )	136.8 ± 20.0^a)⁵ 5) Values are mean ± SD (n = 3). Means with different letters (a-e) in the same column are significantly different at p < 0.05 (Tukey). )	264.2 ± 2.9^d)
D.W	258.2 ± 5.0^de)	615.3 ± 32.9^c)
20% EtOH	238.0 ± 9.3^de)	540.3 ± 23.6^b)
40% EtOH	175.5 ± 8.7^ab)	374.4 ± 12.3^b)
60% EtOH	221.4 ± 21.7^cd)	399.8 ± 14.5^b)
80% EtOH	186.4 ± 15.8^bc)	308.3 ± 8.5^ab)
100% EtOH	280.1 ± 19.7^e)	407.9 ± 12.7^b)

Factors	TPC¹ 1) Total polyphenol contents. )	TFC² 2) Total flavonoid contents. )	PMA³ 3) Phosphomolybdenum complex assay. )	FRAP⁴ 4) Ferric-reducing antioxidant power. )	DPPH	ABTS	NO⁵ 5) Nitric oxide radical scavenging activity. )	HP⁶ 6) Hydrogen peroxide scavenging activity. )
TPC	1	0.784^*⁷ 7) Correlation is significantly different at p < 0.05, **p < 0.01 (Pearson). )	0.553^**	0.664^**	0.433	0.502^**	0.347	0.649^**
TFC		1	0.697^**	0.866^**	0.758^**	0.827^**	0.219	0.408^*
PMA			1	0.678^**	0.298	0.913^**	0.321	0.599^**
FRAP				1	0.743^**	0.165	0.784^**	0.458
DPPH					1	0.562^*	0.349	0.051
ABTS						1	0.122	0.326
NO							1	0.140
HP								1

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[1] *Corresponding author: virshyim@gmail.com