Figure 1
TGF-β1 and IL-1β concentration of difference groups (pg/mg). ***: P < 0.001, compared with NC group; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, compared with Anticoagulant; $: P < 0.05, $$: P < 0.01, compared with Anticoagulant+HSYA-Low; &: P < 0.05, compared with Anticoagulant+HSYA-Middle.
Figure 2
Pathology by HE staining (100×).
Figure 3
CVF of difference groups by Masson staining (100×). ***: P < 0.001, compared with NC group; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, compared with Anticoagulant; $: P < 0.05, $$: P < 0.01, compared with Anticoagulant+HSYA-Low; &: P < 0.05, compared with Anticoagulant+HSYA-Middle.
Figure 4
Collage I and Collage III proteins expression by IHC assay (100×). (A) Collage I protein expression by IHC staining; (B) Collage III protein expression by IHC staining ***: P < 0.001, compared with NC group; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, compared with Anticoagulant; $: P < 0.05, $$: P < 0.01, compared with Anticoagulant+HSYA-Low; &: P < 0.05, compared with Anticoagulant+HSYA-Middle.
Figure 5
TLR4 and NF-κB(p65) protein expression by IHC assay. A. TLR4 protein expression by IHC staining; B. NF-κB(p65) protein express by IHC staining ***: P < 0.001, compared with NC group; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, compared with Anticoagulant; $: P < 0.05, $$: P < 0.01, compared with Anticoagulant+HSYA-Low; &: P < 0.05, compared with Anticoagulant+HSYA-Middle.
Figure 6
Relative mRNA expression by RT-qPCR assay. A. Collage I and Collage III gene expression by RT-qPCR assay ***: P < 0.001, compared with NC group; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, compared with Anticoagulant; $: P < 0.05, $$: P < 0.01, compared with Anticoagulant+HSYA-Low; &: P < 0.05, compared with Anticoagulant+HSYA-Middle; B. TLR4 and NF-κB(p65) gene expression by RT-qPCR assay ***: P < 0.001, compared with NC group; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, compared with Anticoagulant; $: P < 0.05, $$: P < 0.01, compared with Anticoagulant+HSYA-Low; &: P < 0.05, compared with Anticoagulant+HSYA-Middle.
Figure 7
Relative proteins expression by WB assay. (A) Collage I and Collage III proteins expression by WB assay ***: P < 0.001, compared with NC group; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, compared with Anticoagulant; $: P < 0.05, $$: P < 0.01, compared with Anticoagulant+HSYA-Low; &: P < 0.05, compared with Anticoagulant+HSYA-Middle; (B) TLR4 and NF-κB(p65) protein expression by WB assay ***: P < 0.001, compared with NC group; #: P < 0.05, ##: P < 0.01, ###: P < 0.001, compared with Anticoagulant; $: P < 0.05, $$: P < 0.01, compared with Anticoagulant+HSYA-Low; &: P < 0.05, compared with Anticoagulant+HSYA-Middle.
Figure 8
Hyp concentration in difference HYSA treated groups. ***: P < 0.001, compared with NC group; #: P < 0.05; ##: P < 0.01, ###: P < 0.001, compared with Model; $: P < 0.05; $$: P < 0.01, compared with Model+HSYA-L; &: P < 0.05, compared with Model+HSYA-M.
Figure 9
Relative mRNA expressions in difference groups by RT-qPCR assay. ***: P < 0.001, compared with NC group; #: P < 0.05;##: P < 0.01, ###: P < 0.001, compared with Model; $: P < 0.05; $$: P < 0.01, compared with Model+HSYA-L; &: P < 0.05, compared with Model+HSYA-M.
Figure 10
Relative proteins expressions in difference groups by WB assay. ***: P < 0.001, compared with NC group; #: P < 0.05; ##: P < 0.01, ###: P < 0.001, compared with Model; $: P < 0.05; $$: P < 0.01, compared with Model+HSYA-L; &: P < 0.05, compared with Model+HSYA-M.
Figure 11
Collage I protein expression (×200). ***: P < 0.001, compared with NC group; #: P < 0.05;##: P < 0.01, ###: P < 0.001, compared with Model; $: P < 0.05; $$: P < 0.01, compared with Model+HSYA-L; &: P < 0.05, compared with Model+HSYA-M.
Figure 12
Collage III protein expression (×200). ***: P < 0.001, compared with NC group; #: P < 0.05;##: P < 0.01, ###: P < 0.001, compared with Model; $: P < 0.05; $$: P < 0.01, compared with Model+HSYA-L; &: P < 0.05, compared with Model+HSYA-M.
Figure 13
TLR4 protein expression (×200). ***: P < 0.001, compared with NC group; #: P < 0.05;##: P < 0.01, ###: P < 0.001, compared with Model; $: P < 0.05; $$: P < 0.01, compared with Model+HSYA-L; &: P < 0.05, compared with Model+HSYA-M
Figure 14
p-NF-κB(p65) protein nuclear volume (×200). ***: P < 0.001, compared with NC group; #: P < 0.05;##: P < 0.01, ###: P < 0.001, compared with Model; $: P < 0.05; $$: P < 0.01, compared with Model+HSYA-L; &: P < 0.05, compared with Model+HSYA-M.
Figure 15
Hyp concentration. ***: P < 0.001, compared with NC group; ###: P < 0.001, compared with Model.
Figure 16
Relative mRNA expression in difference groups by RT-qPCR assay. ***: P < 0.001, compared with NC group; ###: P < 0.001, compared with Model.
Figure 17
Relative proteins expression in difference groups by RT-qPCR assay. ***: P < 0.001, compared with NC group; ###: P < 0.001, compared with Model.
Figure 18
Collage I protein expression (×200). ***: P < 0.001, compared with NC group; ###: P < 0.001, compared with Model.
Figure 19
Collage III protein expression (×200). ***: P < 0.001, compared with NC group; ###: P < 0.001, compared with Model.
Figure 20
TLR4 protein expression (×200). ***: P < 0.001, compared with NC group; ###: P < 0.001, compared with Model.
Figure 21
p-NF-κB(p65) protein nuclear volume (×200). ***: P < 0.001, compared with NC group; ###: P < 0.001, compared with Model.