Cellulase effect on anaerobic digestion of maize silage under discontinuous operation

Oliva-Merencio, Deny; Pereda-Reyes, Ileana; Schimpf, Ulrike; Koehler, Stefan; Silva, Ariovaldo J. da

doi:10.1590/1809-4430-Eng.Agric.v35n5p951-958/2015

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Scientific Papers • Eng. Agríc. 35 (5) • Sep-Oct 2015 • https://doi.org/10.1590/1809-4430-Eng.Agric.v35n5p951-958/2015 copy

Cellulase effect on anaerobic digestion of maize silage under discontinuous operation

Efeito da adição de enzima (celulase) na digestão anaeróbia de silagem de milho em operação descontínua

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

This paper studied the effect of adding an enzyme (ellulose) on anaerobic digestion of maize silage. We compared materials at chopping lengths of 8 mm (MSL), 4mm (MSS) and natural size (Ms) under a mesophilic and discontinuous operation (batch process). Hence, we found the process to be significantly influenced by particle size. Moreover, the ellulose addition did not significantly impact biogas production after a 35-day digestion period. Ms and MSS displayed an improved response to all variables when compared with MSL and MSL+C, with significant differences. Studies on the refractory fraction at infinite time (R₀) have demonstrated that the lowest values correspond to Ms and MSS (0.122 and 0.155, respectively). The Kinetic approach and the Ultimate Biodegradability test are useful tools to evaluate the effect of the addition of an enzyme to the anaerobic process.

ellulose; silage; biogas; kinetics; biodegradability

Sample		Inoculum	Ms	MSS	MSL	MSL+ C
Mass substrate	(g)	120.00	6.22	5.81	6.34	6.56
TS substrate	(%)	0.03	0.37	0.39	0.36	0.35
VS substrate	(%)	0.64	0.96	0.96	0.96	0.96
Inoculum	(g)	300.00	300.37	300.10	301.17	300.03
Substrate fed	(g)		6.22	5.81	6.34	6.56
Enzyme fed	(mL)					5.0
Total weight	(g)	300.00	306.59	305.91	307.50	311.56
VS ratio		0.00	0.40	0.40	0.40	0.40
VS Substrate	(g)	0.00	2.205	2.205	2.205	2.205

Parameters	Ms	MSS	MSL	MSL+ C
k₀ (d^-1)	0.27 ± 0.007	0.219 ± 0.013	0.247 ± 0.021	0.245 ± 0.016
y_max. (m³CH₄ kg^-1 VS)	0.337 ± 0.002	0.327 ± 0.008	0.305 ± 0.014	0.306 ± 0.013
r_(s)_max. (m³CH₄ kg^-1 VS d^-1)	0.0245 ± 0.0006	0.0188 ± 0.005	0.0191 ± 0.005	0.0154 ± 0.0014
t_Grenz (d)	11.3	13.3	12.2	13.7
UB	0.878	0.845	0.776	0.787

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[TFN1] VS – volatile solids; TS – total solids; Ms – maize silage; MSS – maize silage chopped into 4 mm length; MSL – maize silage chopped into 8 mm length; MSL + C – maize silage added with ellulose.