In this work some vegetables were used as peroxidase source. After the determination of total protein, activity and specific activity, the crude extract of zucchini (Cucurbita pepo) was selected as peroxidase source and used in a flow injection spectrophotometric procedure for the determination of several phenolic compounds such as phenol, catechol, 2,4-dichlorophenol, 4-chloro-3-methylphenol, 4-acetamidophenol, 4-chlorophenol, 2,4,6-trichlorophenol, o-cresol, m-cresol, p-cresol and hydroquinone. After the optimization of the flow injection system, it was used in the determination of phenolic compounds in local industries wastewaters in the concentration range from 2.0x10-4 to 4.0x10-3 mol L-1, with detection limit of 8.0x10-5 mol L-1 and analytical frequency of 58 h-1. The recovery of phenol from three samples ranged from 98.3 to 106.2% and RDS were less than 1.2 % for solutions containing 6.0x10-4 and 8.0x10-4 mol L-1 phenol (n=10).
Crude extract; peroxidase; enzyme; phenolic compounds; wastewater
