Cephaloglycin in B-R buffer pH < 4 is reduced at HMDE very close to the electrolyte discharge (-1.0 V). Nevertheless, in alkaline medium (pH 10) a peak at -0.6V is observed when cephaloglycin is accumulated at accumulation potentials close to +0.20V by formation of an adsorbed mercury complex. The accumulation of this complex is recommended for use in the indirect determination of cephaloglycin by cathodic stripping voltammetry in the range 0.122 to 1.22 mg/mL with pre-concentration for 30 sec at accumulation potential of +0.20V. The detection limit was calculated to be 5,17 ng/mL (n= 3). The relative standard deviation determined from five determinations at the 5x10-7 mol/L level was 1.4 %.
Cephaloglycin; cephalosporins; cathodic stripping voltammetry
