The mycelial growth and sporulation of the Alternaria brasiliensis were evaluated on 20 culture media. Five mm-diameter disks taken from the margins of colonies grown for six days on V-8A3 medium at 25 ºC in the dark were transferred to Petri dishes containing 15 ml of each medium. After seven days, the mycelial growth and the sporulation were evaluated. Although mycelial growth of A. brasiliensis was observed on all tested media, sporulation was registered only on V-8A3, STA5 and SVA6. V-8A3 was the best medium tested in this study for sporulation of the fungus.