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Sporulation of Mycosphaerella fijiensis in different culture media

The conidial production of Mycosphaerella fijiensis (isolate LPM472) was evaluated on seven culture media (potato dextrose agar, V8 agar, V8 CaCO3 agar, coconut water agar, potato carrot agar, banana leaf extract agar and mycophil) under four different light exposure regimes (continuous darkness, 12-hour photoperiod, continuous light and ten days under continuous darkness followed by five days of continuous light). The experiment was arranged using a completely random factorial design with five replications with factor A being the four light regimes and factor B being the seven types of media. An erlenmeyer flask (125 ml) containing 20 ml of culture media and 0,5 ml of conidial suspension (5 x 10(4) conidia/ml) was considered as an experimental unit incubated at 25 ºC + 2 ºC for 15 days. Sporulation was quantified by counting the number of conidia in a Neubaeur chamber. No sporulation was observed under continuous darkness or for the banana leaf extract agar. Under the 12-hour- photoperiod regime, only mycophil and V8 CaCO3 agar provided sporulation while under continuous light, leave in mycophil resulted in sporulation. The best conidial production was obtained on PDA and V8 CaCO3 agar under the ten days of continuous darkness followed by five days of continuous light regime.


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