This study aimed to quantify total phenols and tannins in barks of Anadenanthera peregrina in different diameter classes and compare two methods of quantification of phenols. Barks of thirty-five trees, distributed in seven diameter classes, were collected. They were air-dried, ground and sieved; the material retained on 60 mesh sieve was used. Methanol:water (50%) solution was used for the extraction of phenols. Quantification of total phenols was performed by the modified Folin-Denis and Folin-Ciocalteau methods, while tannins were quantified by the radial diffusion method. No statistically significant differences were found between the phenol quantification methodologies. Increased contents of phenols and tannins were verified as the diameter of trees increased.
barks; phenols; tannins