Abstract
The lignification attenuation process by 2,4-dichlorophenoxiactic (2,4-D) showed efficient to attenuated the lignin metabolism formation. The suspension cells from Eucalyptus urophylla were produced in MS liquid middle supplemented with five different treatments with 2,4-D: (T1: 1 mg/L; T2: 2 mg/L; T3: 4 mg/L; T4: 5 mg/L; T5: 0 mg/L) without antioxidants agent. The infrared and ultraviolet spectrometry were used to determinate of lignin content with cell free of extractive and protein. The treatment 2 with 2 mg/L of 2,4-D provided lowest lignin accumulation (7 mg/L), while in the 5 treatment without 2,4-D was verified the highest lignin content (220 mg/L).
Key words
lignin; Eucalyptus urophylla; cell suspension.