Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis)

ANIMAL GENETICS

SHORT COMMUNICATION

Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis)

Maressa Ferreira Neto^I; Marcelo Ricardo Vicari^II; Edemar Furquim de Camargo^II; Roberto Ferreira Artoni^II; Orlando Moreira-Filho^I

^ILaboratório de Citogenética, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil

^IIDepartamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil

^{Send correspondence to}

ABSTRACT

Cytogenetic data are presented for Astyanax altiparanae populations from three Brazilian hydrographic systems. The chromosomal data obtained in A. altiparanae support the hypothesis of diploid number conservation. However, small differences in the karyotype formula and number of nucleolar organizer regions were observed in these populations. The apparent karyotypical similarity among the studied populations strongly suggests a close relationship among them with some chromosomal divergences due to gene flow restriction.

Key words: cytotaxonomy, karyotype diversification, fish cytogenetics.

Astyanax is a genus from the family Characidae widely distributed throughout South and Central America and previously assigned to the subfamily Tetragonopterinae (Géry, 1977). This group has been recently considered Incertae sedis for not exhibiting consistent evidence of monophyletism (Lima et al., 2003). Astyanax altiparanae is a typical species of the upper Paraná River Basin above Sete Quedas, Brazil (Garutti and Britski, 2000), with the exception of one population from a location in the Iguaçu River situated below this region (Graça and Pavanelli, 2002).

The cytogenetics of Neotropical fishes has greatly contributed to their systematics and taxonomy. There are numerous examples among Astyanax, mainly from the "scabripinnis" and "fasciatus" groups, in which the intraspecific cytogenetic variation among populations was shown to be much larger than that detected through morphological systematics, thereby forming true species complexes (Moreira-Filho and Bertollo, 1991; Artoni et al., 2006; Vicari et al., 2008a).

The aim of this work was to characterize the karyotypes of three A. altiparanae populations and to compare them with those described in the literature. This comparative analysis could reveal population markers and help to establish cytosystematic, evolutionary and/or biogeographical relationships among the populations.

Forty-nine specimens of A. altiparanae were analyzed: nine specimens (five males and four females) from the Pântano Stream in the Tietê River Basin of the state of São Paulo; eight specimens (five males and three females) from the Feijão Stream in the Mogi-Guaçu River Basin in the state of São Paulo; and 32 specimens collected at the Salto Segredo hydroelectric power plant on the Jordão River in the Iguaçu River Basin in the state of Paraná. The procedures were approved by the Ethics Committee on Animal Experimentation of the Universidade Estadual de Ponta Grossa (Process no. 04741/08).

Chromosome preparations were obtained from anterior kidney cells using the in vivo colchicine treatment (Bertollo et al., 1978). Nucleolar organizer regions (NORs) were detected after silver nitrate staining (Ag-NOR) according to Howell and Black (1980) and C-banding was obtained as described in Sumner (1972).

Fluorescent in situ hybridization (FISH) was performed with a biotinyled 18S rDNA probe from the fish Prochilodus argenteus (Hatanaka and Galetti Jr, 2004) and with a biotinyled 5S rDNA probe from the fish Leporinus elongatus (Martins and Galetti Jr, 1999). An Olympus BX50 epifluorescence microscope was used for the analysis.

Nearly 30 metaphases were analyzed per specimen to determine the diploid number, karyotype composition and Ag-NOR distribution. Chromosomes were classified as metacentric (m), submetacentric (sm), subtelocentric (st) and acrocentric (a) following Levan et al. (1964).

Fishes of the genus Astyanax have shown considerable karyotype diversity. The diploid and fundamental numbers (2n and FN) allied to some other chromosome markers, allowed cytotaxonomic and evolutionary inferences regarding these fishes. The A. altiparanae populations studied (Pântano Stream, Feijão Stream and Jordão River) have a diploid number of 2n = 50 chromosomes (Figure 1). However, differences in the fundamental numbers were found among the three populations analyzed. The specimens from the Pântano Stream displayed a FN = 88, with 6m+28sm+4st+12a. The Feijão Stream population had a FN = 94, with 6m+30sm+8st+6a, whereas the specimens from the Jordão River showed a FN = 92, with 6m+28sm+8st+8a (Figure 1, Table 1). In the Jordão River population, chromosome pair 2 was smaller than the same pair in the Pântano and Feijão Streams populations (Figure 1). Variations in diploid number were observed between populations of species from the A. scabripinnis and A. fasciatus complexes and resulted from chromosome translocations (Mantovani et al., 2000; Artoni et al., 2006; Vicari et al., 2008a). Nevertheless, A. altiparanae exhibited a conserved 2n = 50 chromosomes in all the populations karyotyped (Table 1). Despite presenting the same 2n, different fundamental numbers were detected due to chromosome morphology variations. Non-Robertsonian rearrangements, such as pericentric inversions were suggested to explain the karyotype differences among A. altiparanae populations (Domingues et al. 2007).

After C-banding, heterochromatic blocks were observed in the centromeric or pericentromeric regions of all chromosome pairs and in the telomeric regions of some chromosomes with small differences among the analyzed populations (Figure 1b, d and f). This results are similar to findings previously described for other A. altiparanae populations (Fernandes and Martins-Santos, 2004; Domingues et al., 2007). The small variations in constitutive heterochromatin distribution combined with the large variation in FN found among the A. altiparanae populations (Table 1) supports the hypothesis that this structural variation is not due to the amplification of heterochromatic sequences. Thus, the different FNs observed in A. altiparanae populations, sometimes even within the same hydrographic basin, such as the Tibagi, Paranapanema and Mogi-Guaçu Rivers, must be mainly due to pericentric inversions.

Silver-nitrate staining (Ag-NORs) and FISH with the 18S rDNA probe evidenced a single NOR-bearing pair in the population of the Pântano Stream, while the populations of the Feijão Stream and the Jordão River exhibited multiple terminal signals on different chromosomes (Figure 1, in the insets; and Figure 2a, ^c and ^e). Chromosome rearrangements and transference of ribosomal sites could cause these variations, but transposition events have been suggested as the main mechanism to explain the majority of cases of NORs variability in the genome of these animals (Mantovani et al., 2000; Vicari et al., 2008b). Together with variations in the fundamental number, Ag-NORs are important cytogenetic markers to determine differences among populations in this species (Pacheco et al., 2001; Fernandes and Martins-Santos, 2004; 2006). Domingues et al. (2007) carried out a comparative cytogenetic and morphometric study on A. altiparanae populations from the upper Tibagi and Iguaçu Rivers. These authors found a similar karyotype macrostructure in the populations with differences in the number of major rDNA sites between populations. The Ag-NORs and 18S rDNA FISH data (Table 1) also revealed variations between populations of this species both within the same and from different hydrographic basins.

While variations in the number of 18S rDNA sites occurred between populations of A. altiparanae, only one signal in the proximal long arm of a sm chromosome was observed after FISH with the 5S rDNA probe (Figure 2b, ^d and ^e) (Fernandes and Martins-Santos, 2004; 2006; Domingues et al., 2007; Peres et al., 2008; present study). The 5S rDNA sites seemed to be conserved in the proximal region of the long arm of two chromosome pairs (one m and one a) in populations of the A. scabripinnis complex (Ferro et al., 2001; Almeida-Toledo et al., 2002; Mantovani et al., 2005; Vicari et al., 2008a). In Astyanax sp. D and in A. janeiroensis, the 5S rDNA site was located in the proximal region of the long arm of a single acrocentric pair, similar to what was found in the A. scabripinnis complex (Kantek et al., 2007; Vicari et al., 2008b, respectively). Mantovani et al. (2000) proposed that 5S rDNA sites in the proximal position of the long arm indicated a conserved pattern for these genes in Astyanax. Thus, the chromosomes with 5S rDNA sites would be the same among the A. altiparanae populations studied, but would diverge in morphology and number of 5S rDNA sites in other populations of the genus.

The cytogenetic data from the three A. altiparanae populations studied herein are similar to those from other populations of this species reported in the literature. This observation strengthens the hypothesis of a conserved 2n = 50. Nevertheless, differences in the karyotypic formula and in the number of nucleolar organizer regions were noticed when comparing the data from these populations to those already described for other populations. The apparent karyotype similarity strongly suggests a close relationship among the studied populations, but the small karyotypic differences detected indicate some evolutionary divergence due to gene flow restrictions.

Acknowledgments

The authors are grateful to the IAP (Instituto Ambiental do Paraná) and IBAMA (Instituto Brasileiro do Meio Ambiente) for authorizing the capture of specimens (IBAMA/MMA/SISBIO license number: 15117). This work was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico Processo: 473448/2007-6), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and Fundação Araucária (Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná, Processo: 12938). We also thank Mr. Miguel Airton Carvalho for his collaboration in the field work and in the laboratory.

Received: October 7, 2008; Accepted: June 17, 2009.

Associate Editor: Fausto Foresti

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Publication Dates

• Publication in this collection
  09 Oct 2009
• Date of issue
  2009

History

• Received
  07 Oct 2008
• Accepted
  17 June 2009