Adiponectin promoter polymorphisms are predictors of lipid profile improvement after bariatric surgery

Gasparotto, Aline Simas; Borges, Diego Olschowsky; Zandoná, Marília Remuzzi; Ramos, Mauricio Jacques; Meihnardt, Nelson Guardiola; Mattevi, Vanessa S.

doi:10.1590/1678-4685-GMB-2016-0241

Acessibilidade / Reportar erro

Brasil

Genetics and Molecular Biology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Human and Medical Genetics • Genet. Mol. Biol. 40 (4) • Oct-Dec 2017 • https://doi.org/10.1590/1678-4685-GMB-2016-0241 copy

Adiponectin promoter polymorphisms are predictors of lipid profile improvement after bariatric surgery

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Our aim was to investigate if single nucleotide polymorphisms (SNPs) located in the 5′ regions of leptin (LEP, -2548 G > A, rs7799039), resistin (RETN, -420 C > G, rs1862513) and adiponectin (ADIPOQ, -11391 G > A, rs17300539 and -11377 C > G, rs266729) genes were related to changes in body mass index (BMI) and metabolic variables after bariatric surgery in 60 extremely obese individuals. At baseline, ADIPOQ -11391 A-allele carriers showed higher plasma adiponectin and lower total cholesterol levels when compared to G/G homozygotes. Approximately 32 months post-surgery, a mean reduction of 35% in BMI and an important improvement in metabolic profiles were observed. In addition, for the ADIPOQ -11377 polymorphism, a higher decrease in lipid profile was associated to the C/C genotype. Moreover, individuals bearing the A-C haplotype for the two ADIPOQ SNPs were more prone to show a reduction in low-density lipoprotein levels after bariatric surgery (-43.0% A-C carriers vs. -18.1% G-G carriers, p = 0.019). We did not find any association of leptin and resistin SNPs with the clinical parameters analyzed. In summary, our results indicate that the A-C haplotype is a predictor of better lipid profile post-surgery and the studied SNPs in ADIPOQ gene are associated to changes in metabolic variables in obese individuals.

Keywords:
Adiponectin; polymorphism; obesity; lipid profile; bariatric surgery

Characteristic	^* * Data are shown as mean ± SD, median [interquartile range] or absolute n (%). BMI, body mass index.
Age (years)	42.3 ± 8.9
Women	55 (91.7%)
Euro-descendants	57 (95.0%)
Smoking	4 (6.7%)
Sedentary	50 (83.3%)
Type 2 diabetes	32 (53.3%)
Hypertension	44 (73.3%)
Dyslipidemia	36 (60.0%)
Metabolic syndrome	40 (66.7%)
BMI (kg/m²)	50.7 ± 7.8
Abdominal subcutaneous fat thickness (cm)	6.6 ± 1.8
Leptin (ng/mL)^a a Adipokine levels available for 43 individuals;	37.3 [24.7-47.3]
Adiponectin (ug/mL)^a a Adipokine levels available for 43 individuals;	12.5 [10.2-19.5]
Resistin (ng/mL)^a a Adipokine levels available for 43 individuals;	0.59 [0.43-0.80]

Polymorphism	Genotype (n)	Adipokine^* * Data are expressed as median and interquartile range;	Interquartile range
		Leptin (ng/mL)
LEP -2548 G > A (rs7799039)	G/G (24)	36.4	24.8 – 47.8
	A/G (17)	37.3	22.0 – 50.1
	A/A (12)	39.4	32.6 – 42.3
	p	0.988^a a Kruskal-Wallis test;
		Resistin (ng/mL)
RETN -420 C > G (rs1862513)	C/C (22)	0.64	0.44 – 0.92
	G/G+C/G (28)	0.59	0.42 – 0.76
	p	0.422^b b Mann-Whitney U test; n < 60 due to unsuccessful genotyping of some samples.
		Adiponectin (μg/mL)
ADIPOQ -11391 G > A (rs17300539)	G/G (45)	12.4	1.2 – 17.7
	A/A+A/G (11)	19.5	11.9 – 30.4
	p	0.045^b b Mann-Whitney U test; n < 60 due to unsuccessful genotyping of some samples.
ADIPOQ -11377 C > G (rs266729)	C/C (26)	13.6	10.9 – 18.9
	G/G+C/G (31)	12.5	10.2 – 25.4
	p	0.921^b b Mann-Whitney U test; n < 60 due to unsuccessful genotyping of some samples.

	Baseline	After surgery	Variation	Variation (%)	p	n
BMI (kg/m²)	50.3 ± 1.0	32.8 ± 0.7	-17.5 ± 0.8	-35.0	< 0.001 ^a a Paired samples t-test;	59
Total cholesterol (mg/dl)	202.7 ± 5.1	178.0 ± 3.7	-24.7 ± 5.0	-10.7	< 0.001 ^a a Paired samples t-test;	57
LDL cholesterol(mg/dl)	125.2 ± 4.6	93.5 ± 3.3	-31.8 ± 4.2	-22.6	< 0.001 ^a a Paired samples t-test;	56
HDL cholesterol (mg/dl)	48.8 ± 1.3	66.9 ± 1.9	18.2 ± 1.7	41.2	< 0.001 ^a a Paired samples t-test;	57
Triglycerides (mg/dl)	142.6 ± 8.0	87.5 ± 3.5	-55.1 ± 1.3	-40.3	< 0.001 ^b b Wilcoxon test. Second data collection performed 35 ± 6 months (for BMI) and 32 ± 7 months (for the other parameters) after surgery; n < 60 due to unsuccessful genotyping of some samples. BMI, body mass index; LDL, low-density lipoprotein; HDL, high-density lipoprotein.	56
Glucose (mg/dl)	129.1 ± 7.7	91.8 ± 2.7	-37.3 ± 1.2	-21.8	< 0.001 ^b b Wilcoxon test. Second data collection performed 35 ± 6 months (for BMI) and 32 ± 7 months (for the other parameters) after surgery; n < 60 due to unsuccessful genotyping of some samples. BMI, body mass index; LDL, low-density lipoprotein; HDL, high-density lipoprotein.	58
Glycosylated hemoglobin (%)	6.7 ± 0.3	5.6 ± 0.8	-1.1 ± 0.2	-13.4	< 0.001 ^b b Wilcoxon test. Second data collection performed 35 ± 6 months (for BMI) and 32 ± 7 months (for the other parameters) after surgery; n < 60 due to unsuccessful genotyping of some samples. BMI, body mass index; LDL, low-density lipoprotein; HDL, high-density lipoprotein.	53

	C/C	G/G +C/G	p
Δ LDL %	-37.1 [-40.2 – -20.0]	-18.3 [-32.3 – -4.9]	0.005^a a Mann-Whitney U test;
n	23	30
Δ Cholesterol %	-20.7 [-27.5 – -7.0]	-8.8 [-15.6 – 3.4]	0.038^a a Mann-Whitney U test;
n	23	31
Δ Triglycerides %	-43.1 ± 22.0	-31.1 ± 19.5	0.042^b b t-Test for independent samples; n < 60 due to unsuccessful genotyping of some samples. Δ, variation; LDL, low-density lipoprotein.
n	22	31

Sociedade Brasileira de Genética Rua Cap. Adelmio Norberto da Silva, 736, 14025-670 Ribeirão Preto SP Brazil, Tel.: (55 16) 3911-4130 / Fax.: (55 16) 3621-3552 - Ribeirão Preto - SP - Brazil
E-mail: editor@gmb.org.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Send correspondence to Vanessa Suñé Mattevi. Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite, 245, prédio 1, sala 309, 90050-170 Porto Alegre, RS, Brazil. E-mail: vmattevi@ufcspa.edu.br.