The in vitro multiplication of etiolated nodal segments was evaluated for Ananas comosus var. erectifolius shoots production. Stems were inoculated in the following media: MS without growth regulator; MS + 10 µM of indolbutiric acid (IBA); MS + 10 µM of naphtalenacetic acid (NAA); MS + 10 µM of indolacetic acid (IAA); and stored in darkness at 25±2ºC. 60 days after inoculation, the number of etiolated shoots/stem; number of nodes/etiolated shoot; etiolated shoot length, internode length and total number of nodes/stem were evaluated. The medium MS + 10 µM of NAA showed the highest values of number of etiolated shoots and total number of nodes/etiolated shoot. For shoot regeneration, nodal segments from in vitro etiolated stems with two nodes were inoculated in the following media: MS without growth regulator; MS + 4.44 µM of 6-benzylaminopurine (BA); MS + 8.88 µM of BA; MS + 13.32 µM of BA. The flasks were incubated under photoperiod of 16 hours, at 25±2ºC. At 30 days of culture, the number of regenerated shoots/explant did not differ in the tested media. At 45 and 60 days of culture, the media with BAP induced higher number of regenerated shoots per node, differing statistically from the control.
Ananas comosus var. erectifolius; Ananas lucidus; micropropagation; Bromeliaceae; floriculture
