Figs 1-4
Stages of ovarian development of Macrobrachium amazonicum (Heller, 1862Taddei, F. G.; Reis, S. S.; David, F. S.; Silva, T. E.; Fransozo, V. & Fransozo, A. 2017. Population structure, mortality, and recruitment of Macrobrachium amazonicum (Heller, 1862) (Caridea: Palaemonidae) in the eastern Amazon region, Brazil. Journal of Crustacean Biology 37(2):131-141. ) captured in the Cachoeira II reservoir between November 2014 and August 2017. The organ is demarcated by an ellipse: 1, In advanced development; 2, Developed; 3, Spawned similar to an undeveloped organ; 4, Spawned similar to an early developing organ.
Figs 5-10
Photomicrographs of germ cells of the ovary of Macrobrachium amazonicum (Heller, 1862Taddei, F. G.; Reis, S. S.; David, F. S.; Silva, T. E.; Fransozo, V. & Fransozo, A. 2017. Population structure, mortality, and recruitment of Macrobrachium amazonicum (Heller, 1862) (Caridea: Palaemonidae) in the eastern Amazon region, Brazil. Journal of Crustacean Biology 37(2):131-141. ) at different stages of development, captured in the Cachoeira II reservoir between November 2014 and August 2017: 5, Oogonia: nucleus (NU) and nucleolus (NE). The granulations within the nucleus characterize the chromatin; 6, Previtellogenic oocyte: cytoplasm (CY) and two nucleoli (NE) within the nucleus; 7, Initial vitellogenic oocyte: formation of cytoplasmic vesicle (CV) and spherical follicular cells (FC) around the cell; 8, Advanced vitellogenic oocyte: cytoplasmic vesicles (CV) and yolk granules (YG); 9, Mature oocyte: follicular cells (FC) forming a cord-like coating around the oocyte, with center nucleus (NU); 10, Atretic oocyte: showing cellular disorganization. Color: Hematoxylin-Eosin (5, 6, 9, 10); Gomori trichrome (7, 8).
Figs 11, 12
Graphical representation of reproductive cells of Macrobrachium amazonicum (Heller, 1862Taddei, F. G.; Reis, S. S.; David, F. S.; Silva, T. E.; Fransozo, V. & Fransozo, A. 2017. Population structure, mortality, and recruitment of Macrobrachium amazonicum (Heller, 1862) (Caridea: Palaemonidae) in the eastern Amazon region, Brazil. Journal of Crustacean Biology 37(2):131-141. ) females captured in the Cachoeira II reservoir between November 2014 and August 2017: 11, cells diameter; 12, nuclei diameter, showing maximum observation (upper bar), minimum observation (lower bar), third quartile (top of the box), first quartile (bottom of box) and average (inner bar) (OO, oogonia; PVO, previtellogenic oocyte; VO, vitellogenic oocyte; MO, mature oocyte). Horizontally, different letters indicate statistical difference (p<0.01), verified by the Tukey test.
Figs 13-18
Ovary sections of Macrobrachium amazonicum (Heller, 1862Taddei, F. G.; Reis, S. S.; David, F. S.; Silva, T. E.; Fransozo, V. & Fransozo, A. 2017. Population structure, mortality, and recruitment of Macrobrachium amazonicum (Heller, 1862) (Caridea: Palaemonidae) in the eastern Amazon region, Brazil. Journal of Crustacean Biology 37(2):131-141. ) at different maturation stages, captured in the Cachoeira II reservoir between November 2014 and August 2017: 13, Immature: oogonia (OO), previtellogenic oocyte (PVO) and follicular cells (between { }); 14, In maturation: previtellogenic oocyte (PVO), initial vitellogenic oocyte (VO I), advanced vitellogenic oocyte (VO II), and cytoplasmic vesicle formation (CV). Clear nucleus and nucleolus (between { }), and spherical follicular cell nodes (star), also around the oocytes; 15, Mature: follicular cells forming a lining around the oocytes, where they are barely perceptible due to their level of proximity and flattening (FC), and small nucleus (NU). The crystallized appearance is due to the accumulation of yolk granules, and the several small uncolored spots characterize the cytoplasmic vesicles; 16, Spawned in maturation: oogonia displaying their nuclei and nucleoli (OO), previtellogenic oocyte (PVO), initial vitellogenic oocyte (between [ ]), advanced vitellogenic oocyte (between { }) and postovulatory follicles (POF); 17, Spawned: oogonia (OO), atretic oocyte (AO) and postovulatory follicle (POF); 18, Resting: oogonia (OO) and follicular cell nodes (star). Color: Gomori trichrome (13, 14, 16, 18); Hematoxylin-Eosin (15, 17).
Figs 19-24
Distribution of reproductive cell diameters of Macrobachium amazonicum (Heller, 1862Taddei, F. G.; Reis, S. S.; David, F. S.; Silva, T. E.; Fransozo, V. & Fransozo, A. 2017. Population structure, mortality, and recruitment of Macrobrachium amazonicum (Heller, 1862) (Caridea: Palaemonidae) in the eastern Amazon region, Brazil. Journal of Crustacean Biology 37(2):131-141. ) females captured in the Cachoeira II reservoir, between November 2014 and August 2017, in different stages of gonadal maturation: 19, Immature; 20, In maturation; 21, Mature; 22, Spawned in maturation; 23, Spawned; 24, Resting. The peaks of elevations in the curves indicate modal groups (N, number of oocytes measured).
Tab. I
Relation of correspondence between macroscopic stage classification of ovarian development of Macrobrachium amazonicum (Heller, 1862Taddei, F. G.; Reis, S. S.; David, F. S.; Silva, T. E.; Fransozo, V. & Fransozo, A. 2017. Population structure, mortality, and recruitment of Macrobrachium amazonicum (Heller, 1862) (Caridea: Palaemonidae) in the eastern Amazon region, Brazil. Journal of Crustacean Biology 37(2):131-141. ) by different authors in different locations (un, unknown; *, stage of reorganized ovaries in a new reproductive cycle, which passes by the 6th stage of the present study, and is therefore presented in this section).