A new, simple and sensitive method has been proposed for the determination of mezlocillin. It is based on the enhancement of the chemiluminescence (CL) emission generated from the oxidation of luminol in alkaline medium by postassium periodate. The optimization of the experimental and instrumental variables affecting the CL enhancement effect has been carried out using flow-injection system. In the optimum conditions, the method is efficient to determine mezlocillin in the linear range of 0.01-30×10-6 g mL-1 with a detection limit (3σ) of 3.0×10-9 g mL-1 and the relative standard deviation (RSD) is 1.0% for 1.0×10-6 g mL-1 mezlocillin (n=11). It has been successfully applied to the mezlocillin determination in commercial preparations, synthetic samples and biological fluids formulations.
chemiluminescence; flow injection; mezlocillin
