<i>In vitro</i> Photoprotective Evaluation and Development of Novel Nanoemulsion with Chromone Derivative

Antunes, Amanda S.; Gouveia, Ana Paula; Diogo, Gabriela M.; Taylor, Jason G.; Sousa, Lucas R. D.; Amparo, Tatiane R.; Perasoli, Fernanda B.; Santos, Orlando D. H. dos; Cazati, Thiago; Vieira, Paula M. A.; Penido, Ricardo G.; Santos, Viviane M. R. dos

doi:10.21577/0103-5053.20210072

Amanda S. Antunes

Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Ana Paula Gouveia

Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Gabriela M. Diogo

Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Jason G. Taylor

Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Lucas R. D. Sousa

Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Tatiane R. Amparo

Escola de Farmácia, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Fernanda B. Perasoli

Escola de Farmácia, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Orlando D. H. dos Santos

Escola de Farmácia, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Thiago Cazati

Departamento de Física, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

https://orcid.org/0000-0002-3769-9658

Paula M. A. Vieira

Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

Ricardo G. Penido

Departamento de Química, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte-MG, Brazil

Viviane M. R. dos Santos ^**e-mail: vivianesantos@ufop.edu.br

Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto-MG, Brazil

https://orcid.org/0000-0002-0144-9518

*e-mail: vivianesantos@ufop.edu.br

Author Contributions

Amanda S. Antunes, Ricardo G. Penido, Ana Paula Gouveia and Gabriela M. Diogo were responsible for data curation, investigation, synthesis and characterization of organic compounds; Jason G. Taylor for conceptualization, data curation, investigation, validation; Orlando D. H. dos Santos and Fernanda B. Perasoli for formal analysis funding acquisition and validation; Paula M. A. Vieira, Lucas R. D. Sousa, Tatiane R. Amparo for data curation and resources; Thiago Cazati for data curation, formal analysis funding acquisition, investigation, resources, validation and writing original draft; Viviane M. R. dos Santos for investigation, project administration resources, writing original draft and writing-review and editing.

	Nanoemulsion with 3a/ %	Nanoemulsion with 3b/ %	Nanoemulsion with S1 / %	Nanoemulsion with S2 / %	Phase
Span 120-LQ-(MH)	4	4	4	4	O
Ultramona RH 400	6	6	6	6	O
Licuri oil	10	10	10	10	O
Distilled water	q.s.p	q.s.p	q.s.p	q.s.p	A

Concentration / (mg mL⁻¹)	SPF-UVB compounds
Concentration / (mg mL⁻¹)	3a	3b	3c	S1	S2
0.025	10.728 ± 0.004^a a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	11.035 ± 0.012^b a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	5.892 ± 0.010^c a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	34.912 ± 0.063^d a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	10.774 ± 0.049^a a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.
0.030	11.787 ± 0.004^a a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	10.157 ± 0.018^b a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	8.856 ± 0.003^c a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	35.264 ± 0.053^d a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	11.607 ± 0.150^a a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.
0.050	14.053 ± 0.015^a a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	22.515 ± 0.007^b a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	11.692 ± 0.006^c a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	36.027 ± 0.079^d a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	15.533 ± 0.001^e a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.
0.070	20.312 ± 0.003^a a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	26.528 ± 0.008^b a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	14.157 ± 0.011^c a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	36.492 ± 0.059^d a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	17.596 ± 0.004^e a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.
0.100	27.436 ± 0.032^a a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	28.844 ± 0.044^b a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	16.775 ± 0.002^c a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	36.817 ± 0.090^d a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.	19.731 ± 0.006^e a-eIndicate p <0.05 at the same line by one-way ANOVA test; SPF: solar protection factor; chromones: 3a-3c; S1 (standard 1): Neo Heliopan®; S2 (standard 2): benzophenone-3; results expressed as mean ± standard deviation.

Nanoemulsion	Average size ± SD / nm	PI ± SD	Zeta potential ± SD / mV
3a	144.40 ± 0.31	0.28 ± 0.03	-36.00 ± 1.16
3b	148.00 ± 0.70	0.26 ± 0.02	-36.70 ± 1.50
S1	135.60 ± 1.57	0.20 ± 0.01	-30.90 ± 1.94
S2	433.70 ± 46.36	0.57 ± 0.04	-35.70 ± 1.85

Concentration / (mg mL⁻¹)	Nanoemulsion
Concentration / (mg mL⁻¹)	S1	S2	3a	3b
0.025	36.348 ± 0.001^a	11.130 ± 0.008^b	12.894 ± 0.001^c	13.889 ± 0.001^d
0.030	36.917 ± 0.001^a	13.118 ± 0.001^b	19.561 ± 0.001^c	23.745 ± 0.051^d
0.050	38.508 ± 0.002^a	13.343 ± 0.001^b	24.560 ± 0.001^c	27.882 ± 0.037^d
0.070	43.492 ± 0.001^a	20.769 ± 0.001^b	35.439 ± 0.006^c	37.938 ± 0.003^d
0.100	45.115 ± 0.002^a	22.954 ± 0.004^b	37.98 ± 0.001^c	38.555 ± 0.002^d

[1] *e-mail: vivianesantos@ufop.edu.br

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In vitro Photoprotective Evaluation and Development of Novel Nanoemulsion with Chromone Derivative