Nucleic acids and carbohydrates (C–O–C skeletal modes) |
916 |
Proteins (C–N stretch) |
1083 |
Guanine/cytosine, chitin and proteins (N–H bending) |
1259 |
Chitin and proteins (N–H bending) |
1265 |
Lipids (C–H wagging/twisting) |
1300 |
Lipids, proteins and carbohydrates (CH2 and CH3 asymmetric deformation) |
1440 |
Proteins (amide, C=O stretch) |
1654 |
Lipids (ester, C=O stretch) |
1740 |
Minor peaks |
Wavenumber / cm-1
|
Glycerol (C–O–C bending) |
607 |
Proteins (C–C twisting) |
621 |
Tyrosine (C–C twisting) |
643 |
Nucleic acids (C–C ring breathing modes and C−S stretch) |
666-680 |
Amino acid methionine (C–S stretch) |
700 |
Nucleic acids |
720 |
Protein (C–S stretch, CH2 rocking) and adenine (CH2 rocking) |
726 |
Phosphatidylserine and thiocyanate (C–S stretch) |
733 and 737 |
Nucleic acids (thymin ring breathing mode) |
747 |
Proteins (tryptophane ring breathing and bending modes) |
756-760 |
Nucleic acids (phosphodiester O−P−O stretch and ring breathing modes in DNA) |
777-789 |
Phosphate groups (O–P–O stretch and phosphate ion interations) |
798 |
Nucleic phosphodiesters and proline, hydroxyproline, tyrosine and phosphate in nucleic acids (O−P−O stretch and C−C stretch) |
808 and 815 |
DNA, tyrosine (C–C out-of-plane ring breathing, phosphate stretch in DNA) |
815-828 |
Amine deformation |
837 |
Saccharides (C–O–C skeletal modes) |
845 |
Proline and tyrosine (C–C ring breathing modes) |
853 |
Protein and polisaccharides (C–C stretch in amino acid side chain of proline, hydroxyproline and collagen backbone) |
857 |
RNA (C–C stretch, skeletal and ring breathing modes) |
867 |
Saccharides and proteins (C−O−C tryptophane and carbohydrate ring modes and CH2 bending modes in proteins) |
877-884 |
Protein (C–C stretch), saccharides (C−O−C skeletal modes) and nucleic acids (C−O−C ribose vibration and P−O stretch) |
884-897 |
RNA (C–O–C skeletal modes of ribose) |
916 |
Carbohydrates (C−C skeletal modes) |
931 |
Polysaccharides (C−C skeletal modes) |
941 |
α-Helix protein (CH3 symmetrical stretch) |
951 |
Phosphate (P–O stretch) and proteins modes (unassigned) |
960-965 |
C–C wagging |
971 |
Protein β-sheet (C–C stretch) and lipids (=C–H bending) |
980 |
Ribose (C–O stretch) and carbohydrates (C–C stretch) |
994 |
Chitin (C–C ring breathing) and phenylalanine (aromatic C–C stretch) |
1003 |
Solid carbohydrate (C–O stretch) |
1015 |
Protein, phenylalanine and carbohydrates (C–C stretch, C–H bending and C–N stretch) |
1030 |
Phosphate symmetrical stretch |
1044 |
Glycogen (C–C stretch) |
1049 |
Chitin and proteins (C–C and C–N stretches) |
1052 |
Proline, collagen (skeletal C–C stretch) |
1066 |
Phospholipids, nucleic phosphodiester, carbohydrates and proteins (C–C and C–O stretches and O–P–O symmetric stretch) |
1074-1115 |
Carbohydrates and proteins (C–C, C–N and C–O stretches) |
1123-1140 |
Proteins (C–C and C–N stretches) |
1158 |
Protein/amino acids (C–H bending) |
1174 |
DNA nitrogenous bases (C–N stretch) and phosphates (anti-symmetric modes) |
1184-1197 |
Chitin and proteins (C–C and C–N stretches, amide III and C–H bending) |
1198-1204 |
Phosphate, lipid, amide, amino acids, nucleic, amine, carbohydrate and chitin complex (C–C and C–N stretches, CH2 rocking and wagging, =C–H bending, O–P–O anti-symmetric modes, N–H bending and amide III in α-helix and β-sheet protein structures) |
1207-1280 |
Ergosterol (CH2 twisting) |
1286 |
Lipids, nitrogenous bases and carbohydrates (C–H bending, CH2 twisting and wagging, CH3 twisting) |
1290-1306 |
Lipids (CH3CH2 twisting) |
1313 |
Proteins (C–H deformation, amide III of α-helix) |
1321 |
Phospholipids (C−H deformation) |
1328 |
Protein, DNA, lipids, ergosterol and carbohydrates (C−H deformations, wagging and twisting) |
1333-1354 |
Tryptophane (C−H deformations) |
1357 and 1365 |
Saccharides, DNA and lipids (C–H deformation and ring breathing modes) |
1370-1385 |
Lipids, amino acid chains and DNA/RNA (CH2 rocking, C−N stretch, C=O symmetric stretch, N–H in plane deformation) |
1370-1385 |
COO- symmetric stretch and C=C stretch |
1411 and 1417 |
Proteins, lipids, DNA/RNA and carbohydrates (C−H, CH2 and CH3 deformation, scissoring, bending and stretching and N–H in plane deformation) |
1411 and 1417 |
Nitrogenous bases ring breathing and -NH3+ modes |
1485 |
Nucleic acid, amino acids and carotenoids (C=C stretching, N–H bending) |
1499-1516 |
Carotenoid (in plane vibrations of C=C and –C=C– conjugates) |
1524-1528 |
Amide, amino acids, tryptophane and tyrosine (C=C stretch, amide III, N–H deformation and amide II) |
1524-1528 |
Nucleic acids modes (ring breathing C–C stretch) |
1570-1580 |
Phenylalanine and olefin (C=C stretching and bending) |
1584 |
Hydroxyproline and phenylalanine (N–H bending and C=C stretch) |
1589 |
C=N and C=C stretches |
1593 |
Complex organization of protein, α-helix, β-sheets, DNA, lipids, chitin and carbohydrates (C=O stretch, C=C deformation and stretching, N–H bending, ring breathing modes, stacking interactions and hydrogen bond-sensitive organization in peptides) |
1600-1800 |
Glutamic and aspartic acids, amino acids, ester groups in lipids (COO- stretch) |
1600-1800 |
Phospholipids, polysaccharides and pectin (C=O stretch) |
1745 |