Fourier transform infrared spectroscopy (FTIR) based methods for the determination of two anti-diabetic drugs, i.e., metformin HCl and glimepiride in tablet formulation are described. The methods involve the use of carefully prepared KBr discs with standards and samples of metformin HCl and glimepiride. FTIR spectra were acquired in the mid IR region. Extraction and pre concentration procedures were totally avoided. For quantification of metformin HCl, a prominent IR band appearing at fixed location height 3173 cm-1 with two point baseline between 3550-2850 cm-1 and for glimepiride IR band at fixed location height 1708 cm-1 with two point baseline between 1800-1600 cm-1 were selected for Beer's law calibrations. For metformin HCl, calibrations were developed using standards in the range between 0.300 mg to 3.100 mg whereas for glimepiride, calibrations were developed using standards in the range between 0.400 mg to 2.450 mg. Accuracy and reproducibility of proposed methods were evaluated and found to be satisfactory with excellent regression coefficients, acceptable recoveries and low standard deviations. The FTIR methods were successfully applied for the quantification of pharmaceutical tablets formulation containing metformin HCl and glimepiride as active substances and the results were found to be comparable with the quoted values.
transmission FTIR spectroscopy; metformin HCl; glimepiride; tablet formulations
