Neutrophils phagocytosing fungal hyphae in urinary sediment

The Phagocytosis of fungal structures by neutrophils is a well-documented function of these immune cells. However, neutrophil phagocytosis of hyphal structures in the urine sediment is not usually observed during routine sample evaluation. This is a case of hyphal phagocytosis by neutrophils in the urine of a kidney allograft recipient patient.

Keywords:
Phagocytosis; Hyphae; Urinalysis; Neutrophils.

Authorship

José Antonio Tesser Poloni Correspondence to: José Antonio Tesser Poloni. E-mail: josetp@unisinos.br

Universidade do Vale do Rio dos Sinos, Escola de Saúde, São Leopoldo, RS, Brasil.Universidade do Vale do Rio dos SinosBrazilSão Leopoldo, RS, BrazilUniversidade do Vale do Rio dos Sinos, Escola de Saúde, São Leopoldo, RS, Brasil.

Controllab, Rio de Janeiro, RJ, Brasil.ControllabBrazilRio de Janeiro, RJ, BrazilControllab, Rio de Janeiro, RJ, Brasil.

https://orcid.org/0000-0002-7334-5128

Clotilde Druck Garcia

Santa Casa de Misericórdia de Porto Alegre, Serviço de Nefrologia Pediátrica, Porto Alegre, RS, Brasil.Santa Casa de Misericórdia de Porto AlegreBrazilPorto Alegre, RS, BrazilSanta Casa de Misericórdia de Porto Alegre, Serviço de Nefrologia Pediátrica, Porto Alegre, RS, Brasil.

Universidade Federal de Ciências da Saúde de Porto Alegre, Faculdade de Medicina, Porto Alegre, RS, Brasil.Universidade Federal de Ciências da Saúde de Porto AlegreBrazilPorto Alegre, RS, BrazilUniversidade Federal de Ciências da Saúde de Porto Alegre, Faculdade de Medicina, Porto Alegre, RS, Brasil.

https://orcid.org/0000-0002-9014-3819

Liane Nanci Rotta

Universidade Federal de Ciências da Saúde de Porto Alegre, Departamento de Métodos Diagnósticos, Porto Alegre, RS, Brasil.Universidade Federal de Ciências da Saúde de Porto AlegreBrazilPorto Alegre, RS, BrazilUniversidade Federal de Ciências da Saúde de Porto Alegre, Departamento de Métodos Diagnósticos, Porto Alegre, RS, Brasil.

https://orcid.org/0000-0003-1043-5203

Constantin F. Urban

Umeå University, Umeå Centre for Microbial Research, Department of Clinical Microbiology, Umeå, Sweden.Umeå UniversitySwedenUmeå, SwedenUmeå University, Umeå Centre for Microbial Research, Department of Clinical Microbiology, Umeå, Sweden.

https://orcid.org/0000-0003-1438-1134

Correspondence to: José Antonio Tesser Poloni. E-mail: josetp@unisinos.br

SCIMAGO INSTITUTIONS RANKINGS

Controllab, Rio de Janeiro, RJ, Brasil.ControllabBrazilRio de Janeiro, RJ, BrazilControllab, Rio de Janeiro, RJ, Brasil.

Figures

Figures (2)

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Figure 1
(A and B): Fresh and unstained urine sediment viewed in bright field microscopy. Original magnification 400x. At first glance, leukocytes are seen as enlargements of the fungal structure. However, moving carefully the micrometer of the microscope it is possible to note that there are cells (leukocytes) attached to hyphae.

Thumbnail

Figure 2
(A and B): Fresh urine sediment stained with Sternheimer-Malbin stain viewed in bright field microscopy. Original magnification 400x. The use of stain reveals the nuclear structure of the leukocytes and in some of them a trilobular nucleus is clearly seen (white arrows). Neutrophils are the most common leukocyte observed in urine samples and in this case they were firmly attach to hyphal structures.

Figure 1 (A and B): Fresh and unstained urine sediment viewed in bright field microscopy. Original magnification 400x. At first glance, leukocytes are seen as enlargements of the fungal structure. However, moving carefully the micrometer of the microscope it is possible to note that there are cells (leukocytes) attached to hyphae.

Figure 2 (A and B): Fresh urine sediment stained with Sternheimer-Malbin stain viewed in bright field microscopy. Original magnification 400x. The use of stain reveals the nuclear structure of the leukocytes and in some of them a trilobular nucleus is clearly seen (white arrows). Neutrophils are the most common leukocyte observed in urine samples and in this case they were firmly attach to hyphal structures.

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Neutrophils phagocytosing fungal hyphae in urinary sediment

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[1] Correspondence to: José Antonio Tesser Poloni. E-mail: josetp@unisinos.br