There was no statistically significant difference (z-test p = 0.05).
FIPLIQ: formaldehyde-isopropanol-phosphate; SSC: superficial squamous cells; ISC: intermediate squamous cells; PEC: parabasal epithelial cells; MC: metaplastic cells; EC: endocervical cells.
There was no statistically significant difference (z-test p = 0.05).
FIPLIQ: formaldehyde-isopropanol-phosphate; PL: polymorphonuclear leukocytes; HIST: histiocytes; RBC: red blood cells.
FIPLIQ: formaldehyde-isopropanol-phosphate; PL: polymorphonuclear leukocytes; SSC: superficial squamous cells; ISC: intermediate squamous cells; MC: metaplastic cells; EC: endocervical cells.
FIPLIQ: formaldehyde-isopropanol-phosphate; Cspp: Candida spp.; Gv: Gardnerella vaginallis; Lspp: Leptothrix spp.
FIPLIQ: formaldehyde-isopropanol-phosphate.
There was no statistically significant difference (z-test p = 0.05).
FIPLIQ: formaldehyde-isopropanol-phosphate; SSC: superficial squamous cells; ISC: intermediate squamous cells; PEC: parabasal epithelial cells; MC: metaplastic cells; EC: endocervical cells.
There was no statistically significant difference (z-test p = 0.05).
FIPLIQ: formaldehyde-isopropanol-phosphate; PL: polymorphonuclear leukocytes; HIST: histiocytes; RBC: red blood cells.
FIPLIQ: formaldehyde-isopropanol-phosphate; PL: polymorphonuclear leukocytes; SSC: superficial squamous cells; ISC: intermediate squamous cells; MC: metaplastic cells; EC: endocervical cells.
Reagents | Ethanol | Methanol | Acetic acid | Isopropanol | Formaldehyde | Glutaraldehyde |
---|---|---|---|---|---|---|
Concentration measured (ml/dl) | 99.5 97 95 70 77 50 30 20 10 |
1 | 0.3 | 80 70 50 22 20 3 2 1 |
1 0.5 3 |
1 0.5 |
Composition liquid-based | Nucleus stain |
Cytoplasm stain |
Cell preservation |
Cellular overlap |
Artifacts |
---|---|---|---|---|---|
Formaldehyde 1 ml/dl; isopropanol 1 ml/dl; PBS | SA | SA | SA | UN | SA |
Ethanol 20 ml/dl; formaldehyde 1 ml/dl; isopropanol 1 ml/dl; PBS | SA | SA | SA | UN | SA |
Ethanol 20 ml/dl; formaldehyde 3 ml/dl; isopropanol 1 ml/dl; PBS | SA | SA | SA | UN | SA |
Ethanol 50 ml/dl; formaldehyde 1 ml/dl; metanol 1 ml/dl; isopropanol 1 ml/dl; PBS | SA | SA | SA | UN | SA |
Ethanol 10 ml/dl; formaldehyde 1 ml/dl; methanol 1 ml/dl; isopropanol 1 ml/dl; PBS | SA | SA | SA | UN | SA |
Ethanol 50 ml/dl; formaldehyde 1 ml/dl; methanol 1 ml/dl; isopropanol 10 ml/dl; PBS | SA | SA | SA | UN | SA |
Formaldehyde 1 ml/dl; isopropanol 20 ml/dl; PBS | SA | SA | SA | UN | SA |
FIPLIQ | SA | SA | SA | SA | SA |
Parameters | Pap smear | FIPLIQ 0 days | FIPLIQ 3 days | FIPLIQ 7 days | FIPLIQ 15 days |
---|---|---|---|---|---|
Cellular shape | 20 | 20 | 20 | 20 | 20 |
Cell preservation | 20 | 19 | 18 | 19 | 20 |
Chromatin detail | 20 | 19 | 19 | 19 | 20 |
Cellularity | 20 | 20 | 20 | 20 | 20 |
Cytolysis | 20 | 19 | 17 | 19 | 19 |
Cell overlap | 15 | 15 | 19 | 18 | 20 |
Obscured by PMN leukocytes | 20 | 18 | 18 | 18 | 18 |
Obscured by RBC | 20 | 20 | 20 | 20 | 20 |
Alterations | Pap smear | FIPLIQ 0 days | FIPLIQ 3 days | FIPLIQ 7 days | FIPLIQ 15 days |
---|---|---|---|---|---|
Polychromasia | 13 | 16 | 15 | 16 | 16 |
Cytoplasmic vacuolization | 3 | 3 | 3 | 3 | 3 |
Perinuclear halos | 16 | 13 | 10 | 14 | 14 |
Keratohyalin granules | 3 | 3 | 3 | 3 | 3 |
Outermost cytoplasm edges | 9 | 6 | 6 | 8 | 6 |
Koilocytes | 2 | 1 | 1 | 2 | 2 |
Keratosis | 12 | 16 | 11 | 15 | 13 |
Thickening nuclear margin | 2 | 5 | 5 | 5 | 4 |
Kariorrhexis | 2 | 2 | 1 | 1 | 1 |
Nuclear enlargement | 18 | 17 | 17 | 17 | 17 |
Binucleation | 10 | 15 | 16 | 15 | 15 |
Karyopyknosis* * karyopyknosis presence, except in superficial cells. | 12 | 12 | 12 | 10 | 12 |
Lysed nuclei | 2 | 1 | 2 | 3 | 1 |
Hyperchromasia | 9 | 6 | 8 | 8 | 8 |
Increased nucleus/cytoplasm ratio | 7 | 8 | 8 | 8 | 8 |
Karyomegaly | 4 | 4 | 4 | 4 | 4 |
Irregular chromatin clumping | 4 | 2 | 2 | 2 | 2 |