Storage potential of <i>Eugenia uniflora</i> Lam. seeds incubated in different osmotic solutions and temperatures

The ex situ conservation of recalcitrant seeds has been one of the biggest challenges in seed technology. Different strategies have emerged in recent years and among these strategies, osmotic conditioning at controlled temperatures has shown excellent results. Therefore, the objective of this research was to store recalcitrant seeds of E. uniflora through osmotic conditioning in order to reduce the metabolism of these seeds and extend their storability. The seeds were stored at temperatures of 10 and 25 °C in PEG solutions at 0.0 (water), -0.5, -1.0, -1.5, and -2.0 MPa, as well as without moistening, for 3, 6, 12, and 18 months. There was a reduction in metabolism in the treatments with PEG, and seed viability was maintained after 18 months at 25 °C and -1.5 MPa.

Index terms:
conservation; osmoconditioning; recalcitrant seeds

Authorship

Wesley Dias Gomes

Instituto de Pesquisas Ambientais, Av. Miguel Stéfano 3687, São Paulo, SP 04301-902, Brasil. Instituto de Pesquisas AmbientaisBrasilSão Paulo, SP, BrasilInstituto de Pesquisas Ambientais, Av. Miguel Stéfano 3687, São Paulo, SP 04301-902, Brasil.

https://orcid.org/0000-0002-7100-8451

Claudio José Barbedo ^* *Corresponding author cbarbedo@sp.gov.br

https://orcid.org/0000-0002-4417-7495

*Corresponding author
cbarbedo@sp.gov.br

SCIMAGO INSTITUTIONS RANKINGS

Figures | Tables

Figures (4)
Tables (1)

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Figure 1
O₂ consumption, CO₂ release, respiratory quotient (RQ), and oxidative rates of E. uniflora seeds incubated for 12 h at the temperatures of 10 °C (blue) and 25 °C (orange), in the following treatments: control (C), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa. Columns with the same letter do not differ from each other (Tukey’s test, 5%).

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Figure 2
Germination and formation of normal and abnormal seedlings from Eugenia uniflora seeds. A: germinated seed, with emergence of primary root; B: normal seedling with developed shoots and roots; C: abnormal seedling formed during storage; D: abnormal seedling formed during storage but with resumption of shoot development during the germination test; E and F: seeds removed after 12 months of storage in the control, soaked in water (0.0 MPa), or in PEG solutions with different osmotic potentials (-0.5, -1.0, -1.5, and -2.0 MPa) at 10 °C (E) or 25 °C (F).

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Figure 3
Specific germination (%) of E. uniflora seeds after each storage period of 3 (A and B), 6 (C and D), 12 (E and F), and 18 (G and H) months at 10 °C (blue) and 25 °C (orange) in the following treatments: control (C, without moistening), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa.

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Figure 4
Germination percentages (lighter-colored bars) and development of normal seedlings (darker bars) in the germination test of E. uniflora seeds stored for 3 (A and B), 6 (C and D), 12 (E and F), and 18 (G and H) months at 10 °C (blue) and 25 °C (orange) in the following treatments: control (C, without moistening), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa. Columns with the same letter do not differ from each other (Tukey’s test, 5%).

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Table 1
Moisture content and water potential (Ψw) in E. uniflora seeds after removal from BOD chambers at the periods of 3, 6, 12, and 18 months in the following treatments: control (without moistening), water (0.0), and PEG at the potentials of -0.5, -1.0, -1.5, and -2.0 MPa, at 10 and 25 °C.

Figure 1 O₂ consumption, CO₂ release, respiratory quotient (RQ), and oxidative rates of E. uniflora seeds incubated for 12 h at the temperatures of 10 °C (blue) and 25 °C (orange), in the following treatments: control (C), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa. Columns with the same letter do not differ from each other (Tukey’s test, 5%).

Figure 2 Germination and formation of normal and abnormal seedlings from Eugenia uniflora seeds. A: germinated seed, with emergence of primary root; B: normal seedling with developed shoots and roots; C: abnormal seedling formed during storage; D: abnormal seedling formed during storage but with resumption of shoot development during the germination test; E and F: seeds removed after 12 months of storage in the control, soaked in water (0.0 MPa), or in PEG solutions with different osmotic potentials (-0.5, -1.0, -1.5, and -2.0 MPa) at 10 °C (E) or 25 °C (F).

Figure 3 Specific germination (%) of E. uniflora seeds after each storage period of 3 (A and B), 6 (C and D), 12 (E and F), and 18 (G and H) months at 10 °C (blue) and 25 °C (orange) in the following treatments: control (C, without moistening), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa.

Figure 4 Germination percentages (lighter-colored bars) and development of normal seedlings (darker bars) in the germination test of E. uniflora seeds stored for 3 (A and B), 6 (C and D), 12 (E and F), and 18 (G and H) months at 10 °C (blue) and 25 °C (orange) in the following treatments: control (C, without moistening), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa. Columns with the same letter do not differ from each other (Tukey’s test, 5%).

Table 1 Moisture content and water potential (Ψw) in E. uniflora seeds after removal from BOD chambers at the periods of 3, 6, 12, and 18 months in the following treatments: control (without moistening), water (0.0), and PEG at the potentials of -0.5, -1.0, -1.5, and -2.0 MPa, at 10 and 25 °C.

Storage temperature	Type of incubation	Moisture content (%)				Water potential (MPa)
		Period (months)				Period (months)
		3	6	12	18	6	18
10 °C	Control	26	13	12	18	-70.5	-40.1
	0.0	57	55	64	69	-1.2	-1.8
	-0.5	56	53	58	45	-2.1	-3.3
	-1.0	49	51	56	56	-3.4	-3.7
	-1.5	56	55	52	50	-4.2	-4.4
	-2.0	52	51	47	48	-4.6	-5.6
25 °C	Control	19	20	15	18	-44.9	-52.0
	0.0	65	71	58	58	-1.1	-2.0
	-0.5	60	65	51	37	-2.6	-20.8
	-1.0	58	63	55	32	-5.1	-22.6
	-1.5	63	56	31	31	-5.3	-23.5
	-2.0	58	47	44	31	-5.9	-26.2

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Storage potential of <i>Eugenia uniflora</i> Lam. seeds incubated in different osmotic solutions and temperatures

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[1] *Corresponding author
cbarbedo@sp.gov.br