A conservação ex situ de sementes recalcitrantes tem sido um dos maiores desafios da tecnologia de sementes. Diferentes estratégias surgiram nos últimos anos e dentre essas estratégias, o condicionamento osmótico em temperaturas controladas tem demonstrado excelentes resultados. Portanto, o objetivo desta pesquisa foi armazenar sementes recalcitrantes de E. uniflora por meio de condicionamento osmótico, a fim de reduzir o metabolismo dessas sementes e ampliar sua capacidade de armazenamento. As sementes foram armazenadas nas temperaturas de 10 e 25 °C em soluções de PEG a 0,0 (água), -0,5, -1,0, -1,5 e -2,0 MPa e sem umedecimento, durante 3, 6, 12 e 18 meses. Os resultados demonstraram redução do metabolismo nos tratamentos com PEG, e a viabilidade das sementes foi mantida após 18 meses a 25 °C e -1,5 MPa.

Termos para indexação:
conservação; osmocondicionamento; sementes recalcitrantes

Autoria

Wesley Dias Gomes

Instituto de Pesquisas Ambientais, Av. Miguel Stéfano 3687, São Paulo, SP 04301-902, Brasil. Instituto de Pesquisas AmbientaisBrasilSão Paulo, SP, BrasilInstituto de Pesquisas Ambientais, Av. Miguel Stéfano 3687, São Paulo, SP 04301-902, Brasil.

https://orcid.org/0000-0002-7100-8451

Claudio José Barbedo ^* *Corresponding author cbarbedo@sp.gov.br

https://orcid.org/0000-0002-4417-7495

*Corresponding author
cbarbedo@sp.gov.br

SCIMAGO INSTITUTIONS RANKINGS

Figuras | Tabelas

Figuras (4)
Tabelas (1)

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Figure 1
O₂ consumption, CO₂ release, respiratory quotient (RQ), and oxidative rates of E. uniflora seeds incubated for 12 h at the temperatures of 10 °C (blue) and 25 °C (orange), in the following treatments: control (C), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa. Columns with the same letter do not differ from each other (Tukey’s test, 5%).

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Figure 2
Germination and formation of normal and abnormal seedlings from Eugenia uniflora seeds. A: germinated seed, with emergence of primary root; B: normal seedling with developed shoots and roots; C: abnormal seedling formed during storage; D: abnormal seedling formed during storage but with resumption of shoot development during the germination test; E and F: seeds removed after 12 months of storage in the control, soaked in water (0.0 MPa), or in PEG solutions with different osmotic potentials (-0.5, -1.0, -1.5, and -2.0 MPa) at 10 °C (E) or 25 °C (F).

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Figure 3
Specific germination (%) of E. uniflora seeds after each storage period of 3 (A and B), 6 (C and D), 12 (E and F), and 18 (G and H) months at 10 °C (blue) and 25 °C (orange) in the following treatments: control (C, without moistening), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa.

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Figure 4
Germination percentages (lighter-colored bars) and development of normal seedlings (darker bars) in the germination test of E. uniflora seeds stored for 3 (A and B), 6 (C and D), 12 (E and F), and 18 (G and H) months at 10 °C (blue) and 25 °C (orange) in the following treatments: control (C, without moistening), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa. Columns with the same letter do not differ from each other (Tukey’s test, 5%).

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Table 1
Moisture content and water potential (Ψw) in E. uniflora seeds after removal from BOD chambers at the periods of 3, 6, 12, and 18 months in the following treatments: control (without moistening), water (0.0), and PEG at the potentials of -0.5, -1.0, -1.5, and -2.0 MPa, at 10 and 25 °C.

Figure 1 O₂ consumption, CO₂ release, respiratory quotient (RQ), and oxidative rates of E. uniflora seeds incubated for 12 h at the temperatures of 10 °C (blue) and 25 °C (orange), in the following treatments: control (C), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa. Columns with the same letter do not differ from each other (Tukey’s test, 5%).

Figure 2 Germination and formation of normal and abnormal seedlings from Eugenia uniflora seeds. A: germinated seed, with emergence of primary root; B: normal seedling with developed shoots and roots; C: abnormal seedling formed during storage; D: abnormal seedling formed during storage but with resumption of shoot development during the germination test; E and F: seeds removed after 12 months of storage in the control, soaked in water (0.0 MPa), or in PEG solutions with different osmotic potentials (-0.5, -1.0, -1.5, and -2.0 MPa) at 10 °C (E) or 25 °C (F).

Figure 3 Specific germination (%) of E. uniflora seeds after each storage period of 3 (A and B), 6 (C and D), 12 (E and F), and 18 (G and H) months at 10 °C (blue) and 25 °C (orange) in the following treatments: control (C, without moistening), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa.

Figure 4 Germination percentages (lighter-colored bars) and development of normal seedlings (darker bars) in the germination test of E. uniflora seeds stored for 3 (A and B), 6 (C and D), 12 (E and F), and 18 (G and H) months at 10 °C (blue) and 25 °C (orange) in the following treatments: control (C, without moistening), water (0.0), and PEG at the osmotic potentials of -0.5, -1.0, -1.5, and -2.0 MPa. Columns with the same letter do not differ from each other (Tukey’s test, 5%).

Table 1 Moisture content and water potential (Ψw) in E. uniflora seeds after removal from BOD chambers at the periods of 3, 6, 12, and 18 months in the following treatments: control (without moistening), water (0.0), and PEG at the potentials of -0.5, -1.0, -1.5, and -2.0 MPa, at 10 and 25 °C.

Storage temperature	Type of incubation	Moisture content (%)				Water potential (MPa)
		Period (months)				Period (months)
		3	6	12	18	6	18
10 °C	Control	26	13	12	18	-70.5	-40.1
	0.0	57	55	64	69	-1.2	-1.8
	-0.5	56	53	58	45	-2.1	-3.3
	-1.0	49	51	56	56	-3.4	-3.7
	-1.5	56	55	52	50	-4.2	-4.4
	-2.0	52	51	47	48	-4.6	-5.6
25 °C	Control	19	20	15	18	-44.9	-52.0
	0.0	65	71	58	58	-1.1	-2.0
	-0.5	60	65	51	37	-2.6	-20.8
	-1.0	58	63	55	32	-5.1	-22.6
	-1.5	63	56	31	31	-5.3	-23.5
	-2.0	58	47	44	31	-5.9	-26.2

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[1] *Corresponding author
cbarbedo@sp.gov.br