Action of nitric oxide on the physiological potential and biochemical mechanisms of pea seeds

Nitric oxide (NO) can act in biochemical pathways of the germination process; however, there is little information about how it acts on the performance of pea seeds. The aim of this study was to evaluate the physiological and biochemical effects of NO on pea seed germination and vigor. Pea seeds cv. Itapuã 600 obtained from three seed lots with different levels of physiological quality were sown in a substrate moistened with water (control) or sodium nitroprusside (SNP) solution, a NO donor (50 μM), to assess germination, vigor, activity of antioxidant enzymes, reactive oxygen species, lipid peroxidation, and amylase activity. NO application does not alter pea seed germination, but it increases vigor. It is more effective in seeds with lower physiological potential. In addition, NO leads to reduction in oxidative stress, favors the translocation of reserves to the embryo, and has potential for use in the treatment of pea seeds to increase seed vigor.

Index terms:
antioxidant enzymes; germination; lipid peroxidation; sodium nitroprusside

Authorship

Marcelo Coelho Sekita

Universidade Federal de Viçosa (UFV), Departamento de Biologia Vegetal, Campus universitário, Av. PH Rolfs, 36570-900 - Viçosa, MG, Brasil.Universidade Federal de ViçosaBrazilViçosa, MG, BrazilUniversidade Federal de Viçosa (UFV), Departamento de Biologia Vegetal, Campus universitário, Av. PH Rolfs, 36570-900 - Viçosa, MG, Brasil.

https://orcid.org/0000-0003-3730-238X

Denise Cunha Fernandes dos Santos Dias

Universidade Federal de Viçosa (UFV), Departamento de Agronomia, Campus universitário, Av. PH Rolfs, 36570-900 - Viçosa, MG, Brasil.Universidade Federal de ViçosaBrazilViçosa, MG, Brazil Universidade Federal de Viçosa (UFV), Departamento de Agronomia, Campus universitário, Av. PH Rolfs, 36570-900 - Viçosa, MG, Brasil.

https://orcid.org/0000-0002-0596-2490

Daniel Teixeira Pinheiro

https://orcid.org/0000-0002-8060-8790

Aparecida Leonir da Silva

https://orcid.org/0000-0002-1887-4414

Antônio César Batista Matos

Universidade Federal de Viçosa (UFV), Departamento de Engenharia Florestal, Campus universitário, Av. PH Rolfs, 36570-900 - Viçosa, MG, Brasil.Universidade Federal de ViçosaBrazilViçosa, MG, Brazil Universidade Federal de Viçosa (UFV), Departamento de Engenharia Florestal, Campus universitário, Av. PH Rolfs, 36570-900 - Viçosa, MG, Brasil.

https://orcid.org/0000-0002-6917-8961

Laércio Junio da Silva

https://orcid.org/0000-0001-7202-0420

*Corresponding author
E-mail: pinheiroagroufv@gmail.com

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (7)

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Figure 1
Germination (A), first germination count (B), germination speed index (C), and germination percentage in the cold test (D) of three lots of pea seed with or without application of 50 µM SNP. Mean values followed by the same uppercase letter do not differ among the lots (1, 2, and 3) by Tukey’s test at 5% probability. Mean values followed by the same lowercase letter do not differ between the treatments [water (control) and SNP] by the F test at 5% probability. Bars: standard deviation.

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Figure 2
Hypocotyl length (A), radicle length (B), hypocotyl dry matter (C), and radicle dry matter (D) of seedlings coming from three lots of pea seeds with or without application of 50 µM SNP. Mean values followed by the same uppercase letter do not differ among the lots (1, 2, and 3) by Tukey’s test at 5% probability. Mean values followed by the same lowercase letter do not differ between the treatments [water (control) and SNP] by the F test at 5% probability. Bars: standard deviation.

Thumbnail

Figure 3
Activity of the enzymes superoxide dismutase (SOD) and catalase (CAT) during germination of seeds from Lots 1 (A and B), 2 (C and D), and 3 (E and F) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Thumbnail

Figure 4
Activity of the enzymes ascorbate peroxidase (APX) and peroxidase (POX) during germination of seeds from Lots 1 (A and B), 2 (C and D), and 3 (E and F) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Thumbnail

Figure 5
Content of the superoxide radical (O₂ ^-) and hydrogen peroxide (H₂O₂) during germination of seeds from Lots 1 (A and B), 2 (C and D), and 3 (E and F) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Thumbnail

Figure 6
Content of malondialdehyde (MDA) during germination of seeds from Lots 1 (A), 2 (B), and 3 (C) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Thumbnail

Figure 7
Activity of the enzymes α-amylase and β-amylase during germination of seeds from Lots 1 (A and B), 2 (C and D), and 3 (E and F) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Figure 1 Germination (A), first germination count (B), germination speed index (C), and germination percentage in the cold test (D) of three lots of pea seed with or without application of 50 µM SNP. Mean values followed by the same uppercase letter do not differ among the lots (1, 2, and 3) by Tukey’s test at 5% probability. Mean values followed by the same lowercase letter do not differ between the treatments [water (control) and SNP] by the F test at 5% probability. Bars: standard deviation.

Figure 2 Hypocotyl length (A), radicle length (B), hypocotyl dry matter (C), and radicle dry matter (D) of seedlings coming from three lots of pea seeds with or without application of 50 µM SNP. Mean values followed by the same uppercase letter do not differ among the lots (1, 2, and 3) by Tukey’s test at 5% probability. Mean values followed by the same lowercase letter do not differ between the treatments [water (control) and SNP] by the F test at 5% probability. Bars: standard deviation.

Figure 3 Activity of the enzymes superoxide dismutase (SOD) and catalase (CAT) during germination of seeds from Lots 1 (A and B), 2 (C and D), and 3 (E and F) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Figure 4 Activity of the enzymes ascorbate peroxidase (APX) and peroxidase (POX) during germination of seeds from Lots 1 (A and B), 2 (C and D), and 3 (E and F) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Figure 5 Content of the superoxide radical (O₂ ^-) and hydrogen peroxide (H₂O₂) during germination of seeds from Lots 1 (A and B), 2 (C and D), and 3 (E and F) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Figure 6 Content of malondialdehyde (MDA) during germination of seeds from Lots 1 (A), 2 (B), and 3 (C) of pea with or without application of 50 µM SNP. Bars: standard deviation.

Figure 7 Activity of the enzymes α-amylase and β-amylase during germination of seeds from Lots 1 (A and B), 2 (C and D), and 3 (E and F) of pea with or without application of 50 µM SNP. Bars: standard deviation.

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Action of nitric oxide on the physiological potential and biochemical mechanisms of pea seeds

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[1] *Corresponding author
E-mail: pinheiroagroufv@gmail.com