Rocha, Debora Kelli; Carvalho, Everson Reis; Lima, Renato Ribeiro de; Nardelli, Amanda Carvalho Penido; Andrade, Thaís de; Carvalho, Maria Laene Moreira de; Fernandes, Nilceli

Resumo:

O teste de germinação é exigido pelas normas brasileiras para a comercialização de sementes. O uso de metodologia adequada, que expresse a real qualidade fisiológica das sementes e minimize possível fitotoxidez por produtos químicos é importante para a cadeia produtiva. Nesse contexto, objetivou-se avaliar e validar a metodologia de germinação rolo de papel mais vermiculita (RP+V) para uso em sementes de milho. Utilizou-se dois híbridos, sendo um sensível e outro tolerante ao tratamento de sementes, ambos com diferentes níveis de vigor e tratamentos químicos. Dez laboratórios credenciados pelo Ministério da Agricultura e Pecuária (MAPA) foram selecionados para realização e avaliação do teste de germinação RP+V. Os dados foram submetidos a procedimentos estatísticos para estimar a repetibilidade e a reprodutibilidade e verificar a precisão e robustez do teste. Os dados apresentaram acurácia, robustez e precisão dentro dos limites críticos de 1 e 5%. Os resultados estatísticos para repetibilidade, reprodutibilidade e padrões do teste de Mandel apresentaram exatidão e precisão que estão dentro dos limites críticos de 1 e 5% para plântulas normais avaliadas pela metodologia rolo de papel mais vermiculita. Assim, a metodologia pode ser usada rotineiramente para testar sementes de milho, principalmente para aquelas tratadas com produtos fitossanitários.

Termos para indexação:
tratamento químico; controle de qualidade; reprodutibilidade; substrato; Zea mays L

[1] *Corresponding author
eversoncarvalho@ufla.br

Hybrids	Sensitivity	Lot
Hybrid 1	Not sensitive	Lot 1
Hybrid 1	Not sensitive	Lot 2
Hybrid 2	Sensitive	Lot 3
Hybrid 2	Sensitive	Lot 4

Commercial product	Active ingredient g.L^-1	mL^* *Recommendation for 60,000 seeds.
Commercial product	Active ingredient g.L^-1	Dose	Polymer	Volume of spray solution
Cruiser 350 FS®	Thiamethoxam - 350	120	40	160
Cropstar®	Imidacloprid - 150 + Thiodicarb - 450	350	40	390
Poncho®	Clothianidin - 600	80	40	120
Control	-	0	0	0

Samples	Description
1	Hybrid 1 lot 1 treated with Clothianidin
2	Hybrid 1 lot 1 treated with Thiodicarb + Imidacloprid
3	Hybrid 1 lot 1 treated with Thiamethoxam
4	Hybrid 1 lot 1 control
5	Hybrid 1 lot 2 treated with Clothianidin
6	Hybrid 1 lot 2 treated with Thiodicarb + Imidacloprid
7	Hybrid 1 lot 2 treated with Thiamethoxam
8	Hybrid 1 lot 2 control
9	Hybrid 2 lot 3 treated with Clothianidin
10	Hybrid 2 lot 3 treated with Thiodicarb + Imidacloprid
11	Hybrid 2 lot 3 treated with Thiamethoxam
12	Hybrid 2 lot 3 control
13	Hybrid 2 lot 4 treated with Clothianidin
14	Hybrid 2 lot 4 treated with Thiodicarb + Imidacloprid
15	Hybrid 2 lot 4 treated with Thiamethoxam
16	Hybrid 2 lot 4 control

Laboratories responsible	City/State
KWS Melhoramento e Sementes LTDA	Patos de Minas - MG
Laboratório de Análise de Sementes e Mudas - UFLA	Lavras - MG
Copercampos	Campos Novos - SC
Associação dos Produtores de Mato Grosso - APROSMAT	Rondonópolis - MT
LASO/LANAGRO - MG	Belo Horizonte - MG
Bayer do Brasil LTDA	Uberlândia - MG
Qualiteste Análises Agronômicas LTDA	Uberlândia - MG
Seedcare Institute Syngenta	Holambra - SP
Corteva Agriscience	Itumbiara - GO
Cotrijal	Não-me-toque - RS

Lot	FC PR	G PR	FC PR+V	G PR+V	FC SG	SG	FC CT	F CT
1	93 b	96 a	98 A	98 a	93 a	96 A	87 b	87 b
2	96 ab	96 a	98 A	98 a	96 a	97 A	97 a	97 a
3	66 c	91 b	76 B	88 b	71 b	88 B	75 c	83 b
4	99 a	99 a	99 A	99 a	97 a	98 A	96 ab	100 a

Lot	h-value	Condition	Indication
1	-0.5199	Homogeneous variances	Continuing the analysis
2	1.2809	Homogeneous variances	Continuing the analysis
3	0.1732	Homogeneous variances	Continuing the analysis
4	0.2536	Homogeneous variances	Continuing the analysis

Sample	P-value	Condition	Indication
1	0.3127	Homogeneous variances	Continuing the analysis
2	0.2527	Homogeneous variances	Continuing the analysis
3	0.4055	Homogeneous variances	Continuing the analysis
4	0.9159	Homogeneous variances	Continuing the analysis
5	0.9864	Homogeneous variances	Continuing the analysis
6	0.9083	Homogeneous variances	Continuing the analysis
7	0.2130	Homogeneous variances	Continuing the analysis
8	0.3631	Homogeneous variances	Continuing the analysis
9	0.0656	Homogeneous variances	Continuing the analysis
10	0.3905	Homogeneous variances	Continuing the analysis
11	0.1660	Homogeneous variances	Continuing the analysis
12	0.2927	Homogeneous variances	Continuing the analysis
13	0.2066	Homogeneous variances	Continuing the analysis
14	0.3390	Homogeneous variances	Continuing the analysis
15	0.9432	Homogeneous variances	Continuing the analysis
16	0.0733	Homogeneous variances	Continuing the analysis

A	LABS										CL^* (*) Critical limits calculated by multiplying the repeatability values by a numerical Factor D, obtained from Tukey’s (1977) Table, with degrees of freedom tending to infinity.	SR
	Lrj ₁	Lrj ₂	Lrj ₃	Lrj ₄	Lrj ₅	Lrj ₆	Lrj ₇	Lrj ₈	Lrj ₉	Lrj ₁₀
	Cond	Cond	Cond	Cond	Cond	Cond	Cond	Cond	Cond	Cond
1	5.5	6	8	7	4.7	6.5	8	8	6	10	16.4	2.5
1	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	16.4	2.5
2	3	6	4	7	10	4	6.5	8.5	10	6	16.1	2.5
2	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	16.1	2.5
3	3.5	12	8	5	5.4	11	11.5	5.5	10	9	18.3	2.8
3	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	18.3	2.8
4	4.5	8	6	6.5	7.3	6	8	3	8	10	15.7	2.4
4	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	15.7	2.4
5	3.5	6	4	5	5.4	5.5	4	7	2	3	10.3	1.6
5	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	10.3	1.6
6	4	5.2	4	4	6.6	5	4	4.5	6	7	11.1	1.7
6	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	11.1	1.7
7	3	4	4	3.5	6	7.5	6	7	6	3	12.5	1.9
7	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	12.5	1.9
8	3.5	4	4	4	6.7	3.5	8	8	4	7	13.4	2
8	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	13.4	2
9	4.5	12	10	13.5	6.6	6	16	16	10	19	28	4.2
9	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	28	4.2
10	8	16	16	20	18	25	8.5	21	24	18	41.7	6.3
10	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	41.7	6.3
11	13	12	6	16	10	23.5	12.5	12.5	20	13	32.6	5
11	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	32.6	5
12	16.5	9.6	8	10	12	15.5	7	6.5	12	8	24.9	3.8
12	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	24.9	3.8
13	2	2	2	3.5	2	3.5	2	2.5	2	2	6.36	1
13	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	6.36	1
14	3	4	4	1	2.6	8	2	7	4	5	10.9	1.7
14	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	10.9	1.7
15	3.5	4	4	4	4.6	4	4	4.5	4	4	9.55	1.5
15	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	9.55	1.5
16	1.5	4	6	3.5	4	4	2	2	NA	4	8.8	1.4
16	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	^Re	8.8	1.4

Brasil

Brasil

Validation of the paper roll plus vermiculite (PR+V) germination test methodology for treated corn seeds

Resumo: