A análise de imagens por meio da técnica de raios X vem recebendo grande destaque por ser uma técnica importante para identificação de danos internos em sementes, de forma rápida e precisa, além de ser um método não destrutivo. O objetivo do trabalho foi verificar a utilização de raios x para avaliação da morfologia interna de sementes de espécies de adubos verdes. Sementes de tremoço-branco (Lupinus albus L.), feijão guandu-anão (Cajanus cajan (L.) Millsp.), feijão-de-porco (Canavalia ensiformis (L.) DC.) e estilosantes (Stylosanthes spp.) foram radiografadas, sendo estudadas 200 sementes para cada espécie e contabilizadas as sementes intactas e as com danos mecânicos, danos causados por insetos e tecidos deteriorados, considerando a severidade e localização dos danos. As mesmas sementes foram avaliadas pelo teste de germinação. Concluiu-se que a análise de imagens por meio da técnica de raios X é eficiente na detecção de danos internos em sementes das espécies de adubos verdes estudadas e apresenta relação com os aspectos negativos na germinação.

Termos para indexação:
análise de imagens; germinação; danos mecânicos; radiografia

Autoria

Marina Barros Zacharias ^* *Corresponding author marinazacharias@terra.com.br

Universidade de São Paulo, Escola Superior de Agricultura ''Luiz de Queiroz'', Caixa Postal 9, 13418-900 - Piracicaba, SP, Brasil.Universidade de São PauloBrazilPiracicaba, SP, BrazilUniversidade de São Paulo, Escola Superior de Agricultura ''Luiz de Queiroz'', Caixa Postal 9, 13418-900 - Piracicaba, SP, Brasil.

https://orcid.org/0000-0002-5201-2981

Silvio Moure Cicero

https://orcid.org/0000-0002-0676-4007

Francisco Guilhien Gomes-Junior

https://orcid.org/0000-0001-9620-6270

Victor Augusto Forti

Universidade Federal de São Carlos, Centro de Ciências Agrárias, Rodovia Anhanguera, km 174, 13600-970, Araras, SP, Brasil.Universidade Federal de São CarlosBrazilAraras, SP, BrazilUniversidade Federal de São Carlos, Centro de Ciências Agrárias, Rodovia Anhanguera, km 174, 13600-970, Araras, SP, Brasil.

https://orcid.org/0000-0003-2599-3603

Josué Bispo da Silva

Universidade Federal de Mato Grosso do Sul, Campus de Três Lagoas, Av. Ranulpho Marques Leal, 3484, 79613-000, Três Lagoas, MS, Brasil.Universidade Federal de Mato Grosso do SulBrazilTrês Lagoas, MS, BrazilUniversidade Federal de Mato Grosso do Sul, Campus de Três Lagoas, Av. Ranulpho Marques Leal, 3484, 79613-000, Três Lagoas, MS, Brasil.

https://orcid.org/0000-0002-0867-5913

*Corresponding author
marinazacharias@terra.com.br

SCIMAGO INSTITUTIONS RANKINGS

Figuras | Tabelas

Figuras (4)
Tabelas (5)

Thumbnail

Figure 1
Radiograph of white lupin (Lupinus albus L.) seeds without internal damage (A), resulting in normal seedling (B); damage by stink bug in a region far from the embryonic axis (C), resulting in normal seedling (D); deteriorated tissue (E), resulting in dead seed (F); mechanical damage (G), resulting in dead seed (H).

Thumbnail

Figure 2
Radiograph of dwarf pigeon pea (Cajanus cajan (L.) Millsp.) seeds without internal damage (A), resulting in normal seedling (B); deteriorated tissue (C), resulting in abnormal seedling (D); mechanical damage (E), resulting in dead seed (F).

Thumbnail

Figure 3
Radiograph of jack bean (Canavalia ensiformis (L.) DC.) seeds with no internal damage (A), resulting in normal seedling (B); mechanical damage (C), resulting in abnormal seedling (D); mechanical damage (E), resulting in dead seed (F).

Thumbnail

Figure 4
Radiograph of Stylosanthes (Stylosanthes spp.) seeds without internal damage (A), resulting in normal seedling (B); deteriorated tissue (C), resulting in abnormal seedling (D); deteriorated tissue (E), resulting in dead seed (F).

Thumbnail

Table 1
Percentage of normal and abnormal seedlings, dead seeds and moisture content (%) of a total of 200 seeds of white lupin (Lupinus albus L.), dwarf pigeon pea (Cajanus cajan (L.) Millsp.), jack bean (Canavalia ensiformis (L.) DC.) and Stylosanthes (Stylosanthes spp.) evaluated.

Thumbnail

Table 2
Occurrence (%) of mechanical damage, insect damage and deteriorated tissues in the embryonic axis (E1, E2, E3) and cotyledons (C1, C2, C3) of white lupin (Lupinus albus L.) seeds.

Thumbnail

Table 3
Occurrence (%) of mechanical damage, insect damage and deteriorated tissues in the embryonic axis (E1, E2, E3) and cotyledons (C1, C2, C3) of dwarf pigeon pea (Cajanus cajan (L.) Millsp.) seeds.

Thumbnail

Table 4
Occurrence (%) of mechanical damage, insect damage and deteriorated tissues in the embryonic axis (E1, E2, E3) and cotyledons (C1, C2, C3) of jack bean (Canavalia ensiformis (L.) DC.) seeds.

Thumbnail

Table 5
Occurrence (%) of mechanical damage, insect damage and deteriorated tissues in the embryonic axis (E1, E2, E3) and cotyledons (C1, C2, C3) of Stylosanthes (Stylosanthes spp.) seeds.

Figure 1 Radiograph of white lupin (Lupinus albus L.) seeds without internal damage (A), resulting in normal seedling (B); damage by stink bug in a region far from the embryonic axis (C), resulting in normal seedling (D); deteriorated tissue (E), resulting in dead seed (F); mechanical damage (G), resulting in dead seed (H).

Figure 2 Radiograph of dwarf pigeon pea (Cajanus cajan (L.) Millsp.) seeds without internal damage (A), resulting in normal seedling (B); deteriorated tissue (C), resulting in abnormal seedling (D); mechanical damage (E), resulting in dead seed (F).

Figure 3 Radiograph of jack bean (Canavalia ensiformis (L.) DC.) seeds with no internal damage (A), resulting in normal seedling (B); mechanical damage (C), resulting in abnormal seedling (D); mechanical damage (E), resulting in dead seed (F).

Figure 4 Radiograph of Stylosanthes (Stylosanthes spp.) seeds without internal damage (A), resulting in normal seedling (B); deteriorated tissue (C), resulting in abnormal seedling (D); deteriorated tissue (E), resulting in dead seed (F).

Table 1 Percentage of normal and abnormal seedlings, dead seeds and moisture content (%) of a total of 200 seeds of white lupin (Lupinus albus L.), dwarf pigeon pea (Cajanus cajan (L.) Millsp.), jack bean (Canavalia ensiformis (L.) DC.) and Stylosanthes (Stylosanthes spp.) evaluated.

Species	Normal (%)	Abnormal (%)	Dead (%)	Moisture content (%)
White lupin	80	18	2	6.9
Dwarf pigeon pea	54	18	28	8.0
Jack bean	68	23	9	7.9
Stylosanthes	65	9	26	6.1

Table 2 Occurrence (%) of mechanical damage, insect damage and deteriorated tissues in the embryonic axis (E1, E2, E3) and cotyledons (C1, C2, C3) of white lupin (Lupinus albus L.) seeds.

Embryonic axis	Mechanical damage (%)	Insect damage (%)	Deteriorated tissue (%)
E1 - not observed	65	100	93
E2 - non-severe	13	0	0
E3 - severe	22	0	7
Cotyledons
C1 - not observed	89	98	94
C2 - non-severe	5	2	4
C3 - severe	6	0	2

Table 3 Occurrence (%) of mechanical damage, insect damage and deteriorated tissues in the embryonic axis (E1, E2, E3) and cotyledons (C1, C2, C3) of dwarf pigeon pea (Cajanus cajan (L.) Millsp.) seeds.

Embryonic axis	Mechanical damage (%)	Insect damage (%)	Deteriorated tissue (%)
E1 - not observed	43	100	92
E2 - non-severe	19	0	1
E3 - severe	38	0	7
Cotyledons
C1 - not observed	64	100	80
C2 - non-severe	13	0	8
C3 - severe	23	0	12

Table 4 Occurrence (%) of mechanical damage, insect damage and deteriorated tissues in the embryonic axis (E1, E2, E3) and cotyledons (C1, C2, C3) of jack bean (Canavalia ensiformis (L.) DC.) seeds.

Embryonic axis	Mechanical damage (%)	Insect damage (%)	Deteriorated tissue (%)
E1 - not observed	84	99	92
E2 - non-severe	11	1	3
E3 - severe	5	0	5
Cotyledons
C1 - not observed	85	99	95
C2 - non-severe	2	1	3
C3 - severe	13	0	2

Table 5 Occurrence (%) of mechanical damage, insect damage and deteriorated tissues in the embryonic axis (E1, E2, E3) and cotyledons (C1, C2, C3) of Stylosanthes (Stylosanthes spp.) seeds.

Embryonic axis	Mechanical damage (%)	Insect damage (%)	Deteriorated tissue (%)
E1 - not observed	92	100	79
E2 - non-severe	2	0	6
E3 - severe	6	0	15
Cotyledons
C1 - not observed	95	100	85
C2 - non-severe	1	0	3
C3 - severe	4	0	12

Como citar

copiar

ABRATES - Associação Brasileira de Tecnologia de Sementes Av. Juscelino Kubitschek, 1400 - 3° Andar, sala 31 - Centro,, CEP 86020-000 Londrina/PR - Londrina - PR - Brazil
E-mail: jss@abrates.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Mensagem

[1] *Corresponding author
marinazacharias@terra.com.br