Criopreservação de sementes de tabaco condicionadas fisiologicamente

O condicionamento fisiológico é uma técnica comercial utilizada para aumentar a velocidade e uniformidade de germinação das sementes, porém, a qualidade fisiológica das sementes condicionadas é geralmente afetada negativamente durante o armazenamento. Sementes de tabaco da cultivar BAT 2101 foram utilizadas para investigar se sementes condicionadas podem ser criopreservadas. Foram estudadas as substâncias, o tipo de secagem e o reaquecimento mais adequados durante o condicionamento e a criopreservação de sementes de tabaco. O condicionamento das sementes foi realizado com água, espermidina e nitrato de potássio, e a secagem com sílica gel e solução salina saturada. As sementes foram criopreservadas em nitrogênio líquido por 24 h e reaquecidas em banho-maria por dois e cinco minutos e em temperatura ambiente. Sementes de tabaco condicionadas fisiologicamente com espermidina e água podem ser criopreservadas, sem perda de sua qualidade fisiológica, quando secas rapidamente em sílica gel e reaquecidas em banho-maria por dois minutos.

Termos para indexação:
germinação; emergência; Nicotiana tabacum; reaquecimento; secagem

Autoria

Camila Aparecida Lopes ^**Corresponding author <camilalopes_sjc@hotmail.com>

Departamento de Agricultura, Universidade Federal de Lavras, Caixa Postal 3037, 37200-000 - Lavras, MG, Brasil.Universidade Federal de LavrasBrazilLavras, MG, BrazilDepartamento de Agricultura, Universidade Federal de Lavras, Caixa Postal 3037, 37200-000 - Lavras, MG, Brasil.

Maria Laene Moreira de Carvalho

Ana Cristina de Souza

João Almir Oliveira

Dayliane Bernardes de Andrade

*Corresponding author <camilalopes_sjc@hotmail.com>

SCIMAGO INSTITUTIONS RANKINGS

Tabelas

Tabelas (8)

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Table 1
Water content (%) of tobacco (Nicotiana tabacum) seeds of the cultivar BAT2101 after different forms of priming and drying.

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Table 2
Electrical conductivity (µS. cm⁻¹. g⁻¹) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

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Table 3
First germination count (%) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

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Table 4
Germination (%) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

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Table 5
Germination rate index of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

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Table 6
Initial emergence (%) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

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Table 7
Final emergence (%) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

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Table 8
Emergence rate index of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

Table 1 Water content (%) of tobacco (Nicotiana tabacum) seeds of the cultivar BAT2101 after different forms of priming and drying.

Priming	Drying	Water content (%)
Priming	Drying	After priming	After drying
Water	Slow	55.49	6.20
Water	Fast	55.49	6.10
SPD	Slow	54.51	6.07
SPD	Fast	54.51	5.93
KNO₃	Slow	55.39	6.31
KNO₃	Fast	55.39	6.11
Control	-	-	6.00

Table 2 Electrical conductivity (µS. cm⁻¹. g⁻¹) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

A
Priming	Drying	Reheating
Priming	Drying	Ambient temp	WB: 2 min	WB: 5 min
Water	Slow	181.68 bC	152.95 aB	147.40 aA
SPD	Slow	148.44 aA	221.02 bC	264.62 bC
KNO₃	Slow	551.40 cC	426.02 cA	485.94 cB
Water	Fast	155.00 aB	147.13 aA	237.70 bC
SPD	Fast	165.19 bB	156.38 bA	186.61 aC
KNO₃	Fast	427.34 cA	510.92 cB	545.81 cC
B
Priming	Reheating		Drying
Priming	Reheating		Slow	Fast
Water	Ambient temp		181.68 B	155.00 A
Water	WB: 2 min		152.95 B	147.13 A
Water	WB: 5 min		147.40 A	237.70 B
SPD	Ambient temp		148.44 A	165.19 B
SPD	WB: 2 min		221.02 B	156.38 A
SPD	WB: 5 min		264.62 B	186.61 A
KNO₃	Ambient temp		551.40 B	427.34 A
KNO₃	WB: 2 min		426.02 A	510.92 B
KNO₃	WB: 5 min		485.94 A	545.81 B
CV (%) = 5.73

Means followed by the same lowercase letter in the column and uppercase letter in the row do not differ by Scott-Knott test at 5% probability level.

Table 3 First germination count (%) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

A
Priming	Drying	Reheating
Priming	Drying	Ambient temp	WB: 2 min	WB: 5 min
Water	Slow	84 aB	82 aB	90 aA
SPD	Slow	84 aA	83 aA	65 bB
KNO₃	Slow	67 bA	60 bB	59 cB
Water	Fast	83 bB	90 aA	83 cB
SPD	Fast	90 aA	92 aA	87 bB
KNO₃	Fast	89 aA	92 aA	91 aA
B
Priming	Reheating		Drying
Priming	Reheating		Slow	Fast
Water	Ambient temp		84 A	83 A
Water	WB: 2 min		82 B	90 A
Water	WB: 5 min		90 A	83 B
SPD	Ambient temp		84 B	90 A
SPD	WB: 2 min		83 B	92 A
SPD	WB: 5 min		65 B	87 A
KNO₃	Ambient temp		69 B	89 A
KNO₃	WB: 2 min		60 B	92 A
KNO₃	WB: 5 min		59 B	91 A
CV (%) = 12.49

Means followed by the same lowercase letter in the column and uppercase letter in the row do not differ by Scott-Knott test at 5% probability level.

Table 4 Germination (%) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

A
Priming	Drying	Reheating
Priming	Drying	Ambient temp	WB: 2 min	WB: 5 min
Water	Slow	85 bB	82 bC	92 aA
SPD	Slow	89 aA	88 aA	68 bB
KNO₃	Slow	72 cA	61 cB	61 cB
Water	Fast	89 aB	92 aA	89 cB
SPD	Fast	91 aB	88 bC	96 aA
KNO₃	Fast	91 aA	93 aA	92 bA
B
Priming	Reheating		Drying
Priming	Reheating		Slow	Fast
Water	Ambient temp		85 B	89 A
Water	WB: 2 min		82 B	92 A
Water	WB: 5 min		92 A	89 A
SPD	Ambient temp		89 A	91 A
SPD	WB: 2 min		88 A	88 A
SPD	WB: 5 min		68 B	96 A
KNO₃	Ambient temp		72 B	91 A
KNO₃	WB: 2 min		61 B	93 A
KNO₃	WB: 5 min		61 B	92 A
CV (%) = 12.02

Means followed by the same lowercase letter in the column and uppercase letter in the row do not differ by Scott-Knott test at 5% probability level.

Table 5 Germination rate index of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

A
Priming	Drying	Reheating
Priming	Drying	Ambient temp	WB: 2 min	WB: 5 min
Water	Slow	14.19 aA	10.33 bB	16.17 aA
SPD	Slow	16.29 aA	16.47 aA	11.42 bB
KNO₃	Slow	11.29 bA	10.49 bA	11.17 bA
Water	Fast	14.42 aA	15.12 aA	14.46 aA
SPD	Fast	15.38 aA	15.09 aA	15.73 aA
KNO₃	Fast	15.20 aA	16.16 aA	15.92 aA
B
Priming	Reheating		Drying
Priming	Reheating		Slow	Fast
Water	Ambient temp		14.19 A	14.42 A
Water	WB: 2 min		10.33 B	15.12 A
Water	WB: 5 min		16.17 A	14.46 A
SPD	Ambient temp		16.29 A	15.38 A
SPD	WB: 2 min		16.47 A	15.09 A
SPD	WB: 5 min		11.42 B	15.73 A
KNO₃	Ambient temp		11.29 B	15.20 A
KNO₃	WB: 2 min		10.49 B	16.16 A
KNO₃	WB: 5 min		11.17 B	15.92 A
CV (%) = 14.97

Means followed by the same lowercase letter in the column and uppercase letter in the row do not differ by Scott-Knott test at 5% probability level.

Table 6 Initial emergence (%) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

A
Priming	Drying	Reheating
Priming	Drying	Ambient temp	WB: 2 min	WB: 5 min
Water	Slow	85 aB	83 bB	88 aA
SPD	Slow	84 aB	88 aA	66 bC
KNO₃	Slow	56 bB	57 cB	59 cA
Water	Fast	88 aA	91 aA	89 bA
SPD	Fast	89 aB	92 aA	89 bB
KNO₃	Fast	88 aB	83 bC	93 aA
B
Priming	Reheating		Drying
Priming	Reheating		Slow	Fast
Water	Ambient temp		85 B	88 A
Water	WB: 2 min		83 B	91 A
Water	WB: 5 min		88 A	89 A
SPD	Ambient temp		84 B	89 A
SPD	WB: 2 min		88 B	92 A
SPD	WB: 5 min		66 B	89 A
KNO₃	Ambient temp		56 B	88 A
KNO₃	WB: 2 min		57 B	83 A
KNO₃	WB: 5 min		59 B	93 A
CV (%) = 11.44

Means followed by the same lowercase letter in the column and uppercase letter in the row do not differ by Scott-Knott test at 5% probability level.

Table 7 Final emergence (%) of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

A
Priming	Drying	Reheating
Priming	Drying	Ambient temp	WB: 2 min	WB: 5 min
Water	Slow	87 aB	84 bC	90 aA
SPD	Slow	85 bB	89 aA	67 bC
KNO₃	Slow	59 cB	59 cB	62 cA
Water	Fast	92 aA	92 aA	90 cA
SPD	Fast	92 aA	93 aA	93 aA
KNO₃	Fast	91 aA	84 bC	94 aA
B
Priming	Reheating		Drying
Priming	Reheating		Slow	Fast
Water	Ambient temp		87 B	92 A
Water	WB: 2 min		84 B	92 A
Water	WB: 5 min		90 A	90 A
SPD	Ambient temp		85 B	92 A
SPD	WB: 2 min		89 B	93 A
SPD	WB: 5 min		67 B	93 A
KNO₃	Ambient temp		59 B	91 A
KNO₃	WB: 2 min		59 B	84 A
KNO₃	WB: 5 min		62 B	94 A
CV (%) = 11.26

Means followed by the same lowercase letter in the column and uppercase letter in the row do not differ by Scott-Knott test at 5% probability level.

Table 8 Emergence rate index of primed and cryopreserved tobacco (Nicotiana tabacum) seeds of the cultivar BAT 2101 for the factors priming and reheating (A) and drying (B).

A
Priming	Drying	Reheating
Priming	Drying	Ambient temp	WB: 2 min	WB: 5 min
Water	Slow	16.70 aA	16.47 aA	17.33 aA
SPD	Slow	16.16 aA	17.13 aA	12.14 bB
KNO₃	Slow	11.32 bA	12.87 bA	11.21 bA
Water	Fast	16.78 aA	17.07 aA	17.30 aA
SPD	Fast	17.15 aA	17.64 aA	16.39 aA
KNO₃	Fast	16.54 aA	14.59 bB	16.94 aA
B
Priming	Reheating		Drying
Priming	Reheating		Slow	Fast
Water	Ambient temp		16.70 A	16.78 A
Water	WB: 2 min		16.47 A	17.06 A
Water	WB: 5 min		17.32 A	17.30 A
SPD	Ambient temp		16.16 A	17.15 A
SPD	WB: 2 min		17.13 A	17.64 A
SPD	WB: 5 min		12.14 B	16.39 A
KNO₃	Ambient temp		11.32 B	16.54 A
KNO₃	WB: 2 min		12.87 B	14.59 A
KNO₃	WB: 5 min		11.21 B	16.94 A
CV (%) = 6.50

Means followed by the same lowercase letter in the column and uppercase letter in the row do not differ by Scott-Knott test at 5% probability level.

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Criopreservação de sementes de tabaco condicionadas fisiologicamente

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[1] *Corresponding author <camilalopes_sjc@hotmail.com>