Accelerated aging methodologies for evaluating physiological potential of treated soybean seeds

Santos, Rayssa Fernanda dos; Placido, Henrique Fabrício; Bosche, Leonardo Lemes; Zeni Neto, Hugo; Henning, Fernando Augusto; Braccini, Alessandro Lucca

doi:10.1590/2317-1545v43250605

Abstract:

This work evaluated the efficiency of accelerated aging test to determine the physiological potential in treated soybean seeds with fungicides and insecticides, in addition to analyzing the concordance between the results obtained by different accelerated aging methods with the actual results of the physiological quality in seeds after the storage. The study was divided into two experiments, both with entirely randomized delineation, with four replications. In the first experiment, seed germination was evaluated after imposing some treatments that were arranged in a 3 x 2 x 3 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); and accelerated aging (traditional; saturated NaCl solution; no aging). In the second experiment, seedling emergence was evaluated after applying some treatments that were arranged in a 3 x 2 x 2 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); storage time (0 and 135 days). The accelerated aging methods were efficient to determine the physiological potential of the seeds, in which, the NaCl method presented better results. The physiological potential of soybean seeds decreased throughout storage and the application of chemicals in seed treatment impaired the germination and emergence percentages, regardless of the active ingredient used. The accelerated aging method with NaCl presents greater agreement with the actual storage results.

Index terms:
Glycine max; seed treatment; germination; vigor

[1] *Corresponding author
E-mail: rayssaagro@gmail.com

Active ingredients	Type¹ 1 Type: I: insecticide; F: fungicide. 2 Commercial product dose: mL.100 kg-1 of seeds.	Commercial Dose² 1 Type: I: insecticide; F: fungicide. 2 Commercial product dose: mL.100 kg-1 of seeds.
imidacloprid + thiodicarb	I + I	300
fipronil + thiophanate methyl + pyraclostrobin	I + F+ F	200
Control	-	-

SV	Mean Square
Active ingredients	325.06^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Vigor	3556.06^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Accelerated aging	6766.72^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Active ingredients *Vigor	2.06^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Active ingredients * Accelerated aging	83.22^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Vigor* Accelerated aging	393.72^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Active ingredientsVigor Accelerated aging	90.72^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Residue	39.8
C.V.(%)	3.69

SV	Mean Square
SV	Germination	Emergence
Active ingredients	300.06^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.	727.00^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Storage	3056.02^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.	290.08^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Vigor	295.02^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.	1408.33^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Active ingredients * Storage	134.4^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.	39.58^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Active ingredients *Vigor	187.15^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.	80.33^* * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Storage *Vigor	6.02^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.	36.75^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Active ingredients * Storage *Vigor	109.15^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.	47.25^ns * Significant at 5% probability level by the F test; ns: not significant; SV: Source of variation. C.V.: Coefficient of variation.
Residue	48.19	22.26
C.V. (%)	3.97	2.07

Accelerated aging	Germination (%)
Accelerated aging	Seed Vigor Level
Method	High	Low
Traditional	57 Ba	36 Cb
Saturated NaCl solution	63 Ba	48 Bb
No aging	82 Aa	76 Ab

Active ingredients	Germination (%)
imidacloprid + thiodicarb	58 B
fipronil + thiophanate methyl + pyraclostrobin	59 B
Control	65 A

Brasil

Brasil

Accelerated aging methodologies for evaluating physiological potential of treated soybean seeds

Abstract:

Active ingredients	Emergence (%)
	Seed Vigor Level
	High	Low
imidacloprid + thiodicarb	84 Ba	75 Bb
fipronil + thiophanate methyl + pyraclostrobin	89 Ba	73 Bb
control	96 Aa	88 Ab