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Diagnosis • Mem. Inst. Oswaldo Cruz 97 (6) • Sept 2002 • https://doi.org/10.1590/S0074-02762002000600027 linkcopy

Open-access One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis

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    Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.

    Chlamydia; Polymerase Chain Reaction; diagnostic

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    Instituto Oswaldo Cruz, Ministério da Saúde Av. Brasil, 4365 - Pavilhão Mourisco, Manguinhos, 21040-900 Rio de Janeiro RJ Brazil, Tel.: (55 21) 2562-1222, Fax: (55 21) 2562 1220 - Rio de Janeiro - RJ - Brazil
    E-mail: memorias@fiocruz.br
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