Obtenção de novos <i>Ruscus</i> usando radiação Gama

Ruscus é um arbusto sempre-verde que oferece folhagem verde-escura brilhante, utilizado como componente de buquês e arranjos de flores. Uma das formas mais significativas de obter novas variedades de plantas e plantas ornamentais é induzir mutações por radiação. A radiação Gama é mais comumente usada para obter mutantes em culturas comerciais, como as destinadas à alimentação e as plantas ornamentais. Neste estudo, foram desenvolvidos métodos de cultura de tecidos para a proliferação de Ruscus a partir de rizomas para obtenção de aglomerados de rizomas. Esses aglomerados foram subsequentemente irradiados com raios Gama para obter fenótipos únicos, tais como: filocládios estreitos e alongados modificando a simetria do caule e o hábito de crescimento anão. Esses tipos de Ruscus podem contribuir para a expansão da indústria da floricultura.

Palavras-chave:
plantas rizomatosas; reguladores de crescimento; mutações; novas culturas

Autoria

Yaarit Kutsher

Ornamental Plants and Agricultural Biotechnology, ARO, Volcani Center, Israel.Ornamental Plants and Agricultural BiotechnologyIsraelIsraelOrnamental Plants and Agricultural Biotechnology, ARO, Volcani Center, Israel.

Nir Hazenshprung

Soreq Nuclear Research Center, Israel.Soreq Nuclear Research CenterIsraelIsraelSoreq Nuclear Research Center, Israel.

Orit Amir-Segev

Lianne Merchuk-Ovnat

Dalia Evenor

Moshe Reuveni

https://orcid.org/0000-0002-8762-4179

^*Corresponding author: vhmoshe@agri.gov.il

Author Contribution

M.R.: conceived and designed the experiments; Y.K., O.A.S., L.M.O., N.H., and D.A.: performed the experiments; N.H., performed the radiation exposures. M.R. drafted the paper. All authors critically read and approved the final version of the manuscript.

SCIMAGO INSTITUTIONS RANKINGS

Soreq Nuclear Research Center, Israel.Soreq Nuclear Research CenterIsraelIsraelSoreq Nuclear Research Center, Israel.

Figuras

Figuras (6)

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Figure 1
Shoot regeneration from Ruscus rhizomes. Ruscus buds from sterilized rhizomes placed on agar medium containing BA and NAA and produced multiple small buds (arrowheads in B to D) and those small buds sprouted phylloclades. Shoot meristems initiated from flower buds (C and D).

Thumbnail

Figure 2
The effect of cytokinin and auxin on shoot regeneration from rhizomes and flower buds. A: Variations of BA and NAA affected the number of shoots regenerated from each treatment. B: Comparing the number of shoots regenerated from rhizomes or flower buds on the same medium. The SD for the data in the figure of each data point is shown using JMP statistical program. A Tukey test using the JMP program. The Tukey Test is a post-hoc test based on the ranking range distribution.

Thumbnail

Figure 3
The effect of auxin concentration on shoot rooting. The concentration of IBA, NAA and IAA was varied in the presence 1/2 MS and number of rooted shoots was recorded. Each point is the mean of at least three boxes in each treatment and each box contained five to nine single shoots as starting material. Percent survival + SD for the data point. The SD for the data in the figure of each data point is shown using JMP statistical program.

Thumbnail

Figure 4
The effect of Gamma radiation dose on shoot regeneration. Survival of Ruscus rhizome explants treated with increasing amounts of Gamma radiation. Each point is the mean of at least three plates in each treatment and each plate contained nine single rhizome cluster without the phylloclades as shown in Fig, 1. Percent survival + SD for the data point. The SD for the data in the figure of each data point is shown using JMP statistical program.

Thumbnail

Figure 5
The effect Gamma radiation on shoot regeneration. Rhizome explants treated with increasing amounts of Gamma radiation and the number of shoots regenerated from each treatment recorded over time. At each transfer for three years, the number of shoots was recorded. The SD for the data in the figure of each data point is shown using JMP statistical program. The SD for the data in the figure of each data point is shown using JMP statistical program. A Tukey test using the JMP program. The Tukey Test is a post-hoc test based on the ranking range distribution.

Thumbnail

Figure 6
Photos of regenerated Ruscus plants in the soil. A: Control plants; B and C: plants with elongated phylloclades; D and E: plants with three phylloclade per internode. Arrow head point to the internodes. Bar indicates 2 cm.

Figure 1 Shoot regeneration from Ruscus rhizomes. Ruscus buds from sterilized rhizomes placed on agar medium containing BA and NAA and produced multiple small buds (arrowheads in B to D) and those small buds sprouted phylloclades. Shoot meristems initiated from flower buds (C and D).

Figure 2 The effect of cytokinin and auxin on shoot regeneration from rhizomes and flower buds. A: Variations of BA and NAA affected the number of shoots regenerated from each treatment. B: Comparing the number of shoots regenerated from rhizomes or flower buds on the same medium. The SD for the data in the figure of each data point is shown using JMP statistical program. A Tukey test using the JMP program. The Tukey Test is a post-hoc test based on the ranking range distribution.

Figure 3 The effect of auxin concentration on shoot rooting. The concentration of IBA, NAA and IAA was varied in the presence 1/2 MS and number of rooted shoots was recorded. Each point is the mean of at least three boxes in each treatment and each box contained five to nine single shoots as starting material. Percent survival + SD for the data point. The SD for the data in the figure of each data point is shown using JMP statistical program.

Figure 4 The effect of Gamma radiation dose on shoot regeneration. Survival of Ruscus rhizome explants treated with increasing amounts of Gamma radiation. Each point is the mean of at least three plates in each treatment and each plate contained nine single rhizome cluster without the phylloclades as shown in Fig, 1. Percent survival + SD for the data point. The SD for the data in the figure of each data point is shown using JMP statistical program.

Figure 5 The effect Gamma radiation on shoot regeneration. Rhizome explants treated with increasing amounts of Gamma radiation and the number of shoots regenerated from each treatment recorded over time. At each transfer for three years, the number of shoots was recorded. The SD for the data in the figure of each data point is shown using JMP statistical program. The SD for the data in the figure of each data point is shown using JMP statistical program. A Tukey test using the JMP program. The Tukey Test is a post-hoc test based on the ranking range distribution.

Figure 6 Photos of regenerated Ruscus plants in the soil. A: Control plants; B and C: plants with elongated phylloclades; D and E: plants with three phylloclade per internode. Arrow head point to the internodes. Bar indicates 2 cm.

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Obtenção de novos <i>Ruscus</i> usando radiação Gama

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[1] ^*Corresponding author: vhmoshe@agri.gov.il