The objective of this work was to identify the presence of the genes begomovirus resistence Ty-2 and Ty-3 in tomato accessions of the Banco de Germoplasma de Hortaliças of the Universidade Federal de Viçosa. The oligonucleotides TO302 F/R and FLUW25 F/R were used in PCR analysis to verify the presence of markers associated to the genes Ty-2 and Ty-3, respectively. The Ty-2 gene was observed in heterozygosis in the accession BGH-6881 (Solanum peruvianum), and the Ty-3 gene in homozygosis in the accessions BGH-6878, BGH-6897 (S. lycopersicum), and in heterozygosis in acession BGH-6881. The identification of resistance genes, using PCR analysis, represents an advance for tomato breeding programs in Brazil.
Solanum lycopersicum; Solanum peruvianum; geminivirus; PCR; genetic resources
