The objective of this work was to determine multiplication rates and to estimate the in vitro plantlets production of the banana cultivars Preciosa, Maravilha, Pacovan Ken and Japira, during six subcultures at different BAP concentrations. After the multiplication, the rooted microplants were acclimatized, and the survival was determined after 30 days. Multiplication rates reached 2.3 and 2.1 shoots per explant of 'Preciosa' and 'Maravilha', respectively, and 2.7 shoots per explant for 'Pacovan Ken' and 'Japira', in 4 mg L-1 of BAP. Losses due contamination were 22.4%, and seedlings survival was superior to 96%.
Musa sp.; acclimatization; disease resistance; multiplication rate
